Bacterial Media

170 Results Found

Tryptic Soy Agar

Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.

Terrific Broth, Modified

Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.

Terrific Broth

Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.

Potato Dextrose Agar

Suited for the cultivation of yeasts and molds from dairy products and other foods. It may also be used to induce sporulation in many fungi.

Nutrient Broth

Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.

Malt Extract Agar (MEA)

Malt Extract Agar has been used for years to cultivate fungi and yeast cultures in the sugar industry, in the manufacturing of syrups, soft drinks, and other drinks. It is also recommended in conjunction with other specific media which are included in this manual. A medium for the isolation and enumeration of yeasts and molds. It can be used in cultivation of the strains for microbiological vitamin assays.

Glycerol, Microbiological Grade

Microbiological Grade
Highly purified for use as a fixative, a component in bacterial preservation media and for the isolation and maintenance of a variety of microorganisms.

EY Tellurite Enrichment

Egg Yolk Tellurite Enrichment is a tellurite egg yolk emulsion. To be used with Baird-Parker Agar Base for isolating S. aureus.

Columbia Agar Base

Columbia Agar Base is a highly nutritive general purpose medium for the cultivation of fastidious organisms, especially when supplemented with plain or chocolated blood. Basal Medium Eagle is suited for the isolation and cultivation of non-fastidious and fastidious microorganisms from a variety of clinical and non-clinical specimens. It can also be used as a selective isolation medium by adding antimicrobial agents. Columbia Agar Base is used extensively as a medium base for a variety of culture formulations in medical bacteriology. The haemolytic reactions in blood agar are genuinely defined. The majority of the common pathogenic bacteria, however, grow well without the addition of blood. Columbia Agar Base is used satisfactorily in other formulas supplemented by enrichments and /or various inhibitory agents. With the addition of 5-10% sterile defibrinated sheep, rabbit or human blood and, especially when the patient is receiving antibiotic treatment, addition of 1.0 mL of VCN suspension and 1.0 mL of Pronadisa Polyenrichment to 100 mL of medium. Columbia Agar Base becomes an excellent chocolate agar, which can be used to isolate pathogenic gonococci and meningococci, as good or better than Thayer-Martin Medium.

Follicle Stimulating Hormone

Follicle Stimulating Hormone

Casein Hydrolysate Acid Solution

Casein Hydrolysate Acid Solution

TRANSFORM & GROW BACTERIAL TRA

- No Heat Shock Required - Transform-It™ Solution is Ready-to-Use - Transform Plasmids, Ligations, and Cosmids - Store Competent Cells 10 Weeks or Longer - More Reliable than “Do-it-Yourself” Methods - Easy Procedure with Minimal Hands-On Time

EZ-YEAST TRANSFORMATION KIT

- Simple and fast with minimal hands-on time - No need to make cells competent prior to transformation - High throughput: can be easily adapted to 96-well format - Ideal for simultaneous transformation of library and bait vectors into yeast two-hybrid reporter strains - Transformation efficiency up to 1 x 103 colonies per μg DNA

CIRCLEGROW (STERILE SOLUTION)

Superior Plasmid Yield Through Enhanced Cell Growth Perfect for overnight or weekend cultures, CIRCLEGROW™ Medium is a super-rich bacterial growth medium that produces up to three times more plasmid DNA than traditional growth medium. CIRCLEGROW™ Medium consists of a protein and amino acid source, biological buffer, and a complex carbon source that gets metabolized at a much slower rate than glucose, thus prolonging the cell cycle and preventing cell death during the log phase of growth. Cells grown with CIRCLEGROW™® Medium remain healthy for more than two days and continue to divide slowly after the log phase of the growth has been completed. Reduce Hands-On Work and Preparation Time CIRCLEGROW™ cultures do not need to be constantly monitored by O.D. readings due to the incremental post-log cell growth. Obtain excellent yields of plasmid DNA from smaller culture volumes, and confidently leave cultures overnight or over the weekend. CIRCLEGROW™ capsules are the most convenient form of this unique growth media. Pre-mixed CIRCLEGROW™ is also available in bulk powder, single-use pouches, capsules and sterile liquid.

ALKALI-CATION YEAST TRANSFORMA

The Alkali-Cation™ Yeast Transformation Kit provides the reagents needed to perform an easy and quick transformation of yeast, while obtaining a large number of transformants. Its ease of use, robustness, and scalability make this the method of choice for the high-efficiency transformation needed in two hybrid screens. Host cells are made competent by treatment with a lithium/cesium acetate solution. Plasmid DNA is added to the competent cells along with carefully prepared carrier DNA that has been optimized to provide high transformation effi ciencies. The Alkali-Cation™ Yeast Transformation Kit has also been successfully used to transform linear DNA into P. pastoris for protein expression studies.

Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate

Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originally cultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunn developed a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.

Trace Element Solution 2 (1000×)

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It has the same composition as Cleveland’s Trace Element I.

Trace Element Solution 1 (1000×)

MP Biomedicals offers a 1000X sterile filtered solution for use with serum-free media. It includes selenium, copper, zinc, and iron, as well as ferric citrate for greater stability.