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Bacterial Media, Powder, Mammalian Media

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Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate

Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originally cultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunn developed a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.

Ham's F-10, With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.

High Growth Enhancement Medium, With L-Glutamine, Without Sodium Bicarbonate

High Growth Enhancement Medium is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.

Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate, Sodium Pyruvate

Dulbecco's Modification of Eagle's Medium, with L-Glutamine, without Sodium Bicarbonate, without Sodium Pyruvate

Alpha Modification Of Eagle's Medium (AMEM), Powder, With L-Glutamine, Without Deoxyribosides, Ribosides, Sodium Bicarbonate

Alpha Modification of Eagle's Medium (AMEM), powder, with L-glutamine, without deoxyribosides, ribosides, sodium bicarbonate. Originally used for growth of hamster kidney cells.

Ham's F-12 (Modified) With L-Glutamine, Without Sodium Bicarbonate

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells. MgCl2 has been substituted by MgSO4 containing an equivalent amount of Mg ion.

Hanks' Balanced Salts, Powder, Without Sodium Bicarbonate

Hanks' Balanced Salts solution is used to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Phosphate Buffered Saline (PBS), Dulbecco's Formula, Powder, Without Calcium, Magnesium

Phosphate Buffered Salts, Dulbecco's Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

Minimum Essential Medium Eagle (Modified), With Earle's Salts and L-Glutamine, Without Sodium Bicarbonate

Minimum Essential Medium Eagle (Modified), with Earle's Salts and L-Glutamine, without Sodium Bicarbonate was originally prepared by Harry Eagle and is one of the most popular cell culture media. The formulation differs from the original in that the concentrations of some of the vitamins have been increased slightly; in some, the amino acids differ by decimal amounts. In some formulation, the MgSO4 concentration differs from that of Eagle but agrees with that quoted by Parker (Parker quotes 200.00 mg/liter MgSO4.7H2O).

Medium 199 With Earle's Salt (E199), Powder, With L-Glutamine, Without Sodium Bicarbonate

Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hanks' salts.

Medium 199 With Hanks' Salts (H199), Powder, With L-Glutamine Without Sodium Bicarbonate

Medium 199, originally described by Morgan and his colleagues (1950), is a completely defined nutritional source for cell culture. Their investigations demonstrated that cell growth could be measured in this medium. It has broad species applicability including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle's or Hanks' salts.

Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate

Dulbecco's Modified Eagle's Medium (DMEM) is the most widely used modification of Eagle's Basal Medium (BME). DMEM contains four times greater concentration of amino acids, vitamins and supplementary components. The original formulation calls for 1000 mg/L of glucose, which was first employed to support the polyoma virus in primary and secondary embryonic mouse cultures. For optimal culturing of other cell types, a modification of 4500 mg/L glucose is available.

Biorich W/Gln

BioRich 1 is a DMEM/F-12 powdered media blend enriched with trace elements. This permits the transition to "serum-free" conditions reducing or eliminating the necessity for serum supplementation. It is used with ITS Premix, a completely serum independent.

RPMI 1640, With L-Glutamine, Without Sodium Bicarbonate

This medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

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