Bacterial Media, Powder
Tryptone is a pancreatic digest of casein. Tryptone is utillized as a source of nitrogen in many culture media for growing bacteria as well as fungi. The lack of detectable carbohydrates makes this peptone an excellent choice for bacterial studies based on fermentation reactions. Its high level of tryptophan makes it useful in the production of indol. This preparation supplements media for the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as E. coli and Pseudomonas species. It is used to supplement media in the cultivation of anaerobes (Clostridia and other fermenting organisms, e.g., Lactobacilli).
Tryptic Soy Broth
Tryptic Soy Broth is used for the cultivation of fastidious and non-fastidious microorganisms. The inclusion of casein peptone and soy peptone to the formula enhances the growth of many fastidious microorganisms without the addition of blood or serum. It can support the growth of a wide variety of microorganisms including aerobes, anaerobes and fungi. Because of its nutritional capabilities it can be used as an enrichment broth and as a blood culture medium.
Tryptic Soy Agar
Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.
Terrific Broth, Modified
Terrific Broth, Modified is a buffered enriched medium for the propagation of recombinant E. coli strains. The medium was developed by Tartoff and Hobbs. Recombinant strains have an extended growth phase in the medium. The addition of extra Tryptone and Yeast Extract in the medium allows higher plasmid yield per volume. Glycerol is used as a carbohydrate source in this formulation. Unlike glucose, glycerol is not fermented to acetic acid. Terrific Broth, Modified has a lower concentraion of Dibasic Potassium Phosphate than Terrific Broth.
Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.
Malt Extract Agar (MEA)
Malt Extract Agar has been used for years to cultivate fungi and yeast cultures in the sugar industry, in the manufacturing of syrups, soft drinks, and other drinks. It is also recommended in conjunction with other specific media which are included in this manual. A medium for the isolation and enumeration of yeasts and molds. It can be used in cultivation of the strains for microbiological vitamin assays.
Columbia Agar Base
Columbia Agar Base is a highly nutritive general purpose medium for the cultivation of fastidious organisms, especially when supplemented with plain or chocolated blood. Basal Medium Eagle is suited for the isolation and cultivation of non-fastidious and fastidious microorganisms from a variety of clinical and non-clinical specimens. It can also be used as a selective isolation medium by adding antimicrobial agents. Columbia Agar Base is used extensively as a medium base for a variety of culture formulations in medical bacteriology. The haemolytic reactions in blood agar are genuinely defined. The majority of the common pathogenic bacteria, however, grow well without the addition of blood. Columbia Agar Base is used satisfactorily in other formulas supplemented by enrichments and /or various inhibitory agents. With the addition of 5-10% sterile defibrinated sheep, rabbit or human blood and, especially when the patient is receiving antibiotic treatment, addition of 1.0 mL of VCN suspension and 1.0 mL of Pronadisa Polyenrichment to 100 mL of medium. Columbia Agar Base becomes an excellent chocolate agar, which can be used to isolate pathogenic gonococci and meningococci, as good or better than Thayer-Martin Medium.
Williams Medium E, Powder, With L-Glutamine, Without Sodium Bicarbonate
Isolated epithelial cells, as described in his sequential plating method by Williams, et al., in 1971, were originally cultivated in a rich medium known as Williams' Medium D. From these early newborn animal studies, Williams and Gunn developed a subsequent medium, Williams' Medium E, for the long-term cultivation of adult rat liver epithelial cells.
Potato Dextrose Broth
Potato Dextrose Broth is used for the isolation, enumeration and identification of yeast and molds. It can be used in the analysis of dairy products, bottled drinks, frozen food and other types of food. It can also be used in the identification of fungi and yeasts in parallel with their cellular morphology or in methods of mirocultivation in slides. When the medium is to be used for enumeration of molds and yeasts, add to the medium, sterilized and cooled to 45-50 °C, approximately 14 mL of a sterilized 10% solution of tartaric acid to obtain a pH of 3.5.
Ham's F-10, With L-Glutamine, Without Sodium Bicarbonate
Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells.
High Growth Enhancement Medium, With L-Glutamine, Without Sodium Bicarbonate
High Growth Enhancement Medium is a modification of DMEM in which 3.6 g/liter fructose is substituted for glucose, resulting in better pH control and higher cell yields. Fructose is metabolized slower than glucose, resulting in slower acid buildup.
Dulbecco's Modification Of Eagle's Medium (DMEM), Powder, With 4500 mg/L Dextrose, L-Glutamine, Without Sodium Bicarbonate, Sodium Pyruvate
Dulbecco's Modification of Eagle's Medium, with L-Glutamine, without Sodium Bicarbonate, without Sodium Pyruvate