Cell Culture Reagents
Hybridoma Cell Growth Supplement
From Cultured Human Endothelial Cells
HCGS will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell need.
HCGS may be used in dilutions of 1:5 to 1:10 (v/v). We recommend HCGS be thawed and sterile filtered (0.22 or 0.45 membrane) immediately prior to use.
Interleukin-2, Human, Natural
Human Interleukin-2 (IL-2), or Human T-cell growth factor is a glycoprotein which promotes in-vitro proliferation and long-term growth of T-cell lymphokines. Additionaly, IL-2 stimulates production of cytotoxic lymphocytes, natural killer cells, and lymphokine-activated killer cells.
200 mM L-Glutamine (Frozen)
L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Phenol Red, 0.5% Solution
Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.
Donor Horse Serum
This serum is processed from whole blood collected from a controlled herd of young horses. Each animal is routinely screened for Equine Infectious Anemia (EIA). Donor Horse Serum is suitable for use in diagnostic assays, as a supplement in mycoplasma growth and assay media and for culturing hematopoietic stem cells and neuronal cells.
Endothelial Cell Growth Supplement
From Bovine Brain
Lyophilized powder with streptomycin sulfate.
ECGS is a broad culture medium growth supplement containing several growth factors which supports the growth of mammalian vascular endothelial cells and can be used in replacement of cell feeder layers in support of hybridoma clonal growth. It is isolated from bovine brain by a modification of the method of Maciag.
Ref.: Maciag, et al., Proc. Nat'l. Acad. Sci. USA, 76: 5674-5678 (1979).
The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin.
Amphotericin-B (250 µg/mL) 20 mL
Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols, primarily ergosterols, of fungal and bacterial cell membranes. It is an antibiotic solution effective against yeast and other fungi. Amphotericin B may be toxic to some insect cell types. Amphotericin B, is an antimycotic.
G418 Sulfate Solution
G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast,higher plants, mammalian cells, protozoans and helminths. The resistance genes are located on transposons Tn601(903) and Tn5 and are bacterial in derivation, but they can be expressed in eukaryotic cells. By introducing these genes into cells, resistance to G418 Sulfate is conferred. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.
Gentamicin Sul,10 mg/mL
Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1 (C21H43N5O7), C1a (C19H39N5O), and C2 (C20H41N5O7). Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base. Gentamicin sulfate is a broad spectrum antibiotic. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.
Goat Serum, Heat Inactivated
Goat Serum is used as a supplement to cell culture media. Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Be certain to maintain Good Cell Culture Practice, and maintain sterility of cultures that require media supplementation.