Cell Culture Reagents

59 Results Found
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Amphotericin-B (250 µg/mL) 20 mL

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols, primarily ergosterols, of fungal and bacterial cell membranes. It is an antibiotic solution effective against yeast and other fungi. Amphotericin B may be toxic to some insect cell types. Amphotericin B, is an antimycotic.

G418 Sulfate Solution

G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast,higher plants, mammalian cells, protozoans and helminths. The resistance genes are located on transposons Tn601(903) and Tn5 and are bacterial in derivation, but they can be expressed in eukaryotic cells. By introducing these genes into cells, resistance to G418 Sulfate is conferred. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.

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Gentamicin Sul,10 mg/mL

Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1 (C21H43N5O7), C1a (C19H39N5O), and C2 (C20H41N5O7). Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base. Gentamicin sulfate is a broad spectrum antibiotic. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.

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Gentamicin Sul,50 mg/mL

Gentamicin Reagent Solution is effective against gram-positive and gram-negative bacteria, as well as mycoplasma.

Human Serum, Pooled

This serum is obtained from pooled, normal clotted human blood.

Goat Serum

This serum is produced from whole blood collected from healthy adult animals.

Goat Serum, Heat Inactivated

Goat Serum is used as a supplement to cell culture media. Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Be certain to maintain Good Cell Culture Practice, and maintain sterility of cultures that require media supplementation.

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Phosphate Buffered Saline Tablets (PBS)

These balanced salt tablets are specifically formulated for Immunoassay procedures.

X-GAL (POWDER) (m)

Bulk powder, easily weighed to make custom batch sizes.

Endothelial Cell Growth Supplement

(ECGS)
From Bovine Brain
Lyophilized powder with streptomycin sulfate.
ECGS is a broad culture medium growth supplement containing several growth factors which supports the growth of mammalian vascular endothelial cells and can be used in replacement of cell feeder layers in support of hybridoma clonal growth. It is isolated from bovine brain by a modification of the method of Maciag.
Ref.: Maciag, et al., Proc. Nat'l. Acad. Sci. USA, 76: 5674-5678 (1979).

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PENICILLIN-STREPTOMYCIN 100ML

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 10,000 units/mL of penicillin and 10,000 ug/mL of streptomycin.

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Kanamycin (5000 µg/mL) 50 mL

Kanamycin is an aminoglycoside antibiotic effective against mycoplasma, gram-positive and gram-negative bacteria. It is used in biotechnology applications to inhibit protein synthesis.

Pen-Strep-Amphotericin

This triple antibiotic solution contains penicillin (10,000 IU/mL), streptomycin (10 mg/mL), and amphotericin B (25 μg/mL).

Cellvation Cryopres Medium

Cellvation is a unique cryopreservation medium designed for optimal cell recovery and convenience. It does not contain DMSO or serum, and is scientifically engineered for Serum-Free cultures, as well as, ideally suited for all types of serum. To complete the serum-free product range, MP introduces CellVationtm, a unique cryopreservation medium which does not contain either DMSO or serum. This cell freezing medium offers the following benefits: – No presence of serum during freeze/thaw-cycles of cells adapted to serum-free growth – Absence of cytotoxicity or morphological differentiation associated with DMSO – Optimal recovery and faster cell attachment to substrate – Ready to use CellVation has a proven track record of successful freeze/thaw cycles of over 30 cell types, including primary and finite life cells as well as suspension and anchorage-dependent cell lines.

EDTA Disodium Salt, 0.2% Solution, in Phosphate Buffered Saline without Calcium and Magnesium

EDTA Disodium Salt, 0.2% Solution, in Phosphate Buffered Saline without Calcium and Magnesium

Endothelial Cell Growth Supplement

(ECGS)
From Bovine Hypothalamus
Lyophilized from sodium chloride buffer containing streptomycin sulfate
Activity: promotes the growth of Fetal Bovine Heart Endothelial Cells (FBHEC) in 10% newborn calf serum when reconstituted at a concentration of 50-150 μg/ml serum-free medium.
It contains several growth factors (e.g. FGF, ECGF-α) and supports the growth of vascular endothelial cells from human and other mammalian species. Also, it may be used for the cloning and proliferation of hybridomas and human keratinocytes.
Each lot is sterile filtered and tested for bacteria, fungi, and mycoplasma contamination. Some cells may require supplementation with 1-100 μg/ml heparin.

Epidermal Growth Factor 95%

Epidermal growth factor or EGF is a growth factor isolated from mouse submaxillary glands that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells.