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Cell Culture Reagents, Liquid, Cryopreservation Media

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Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Kanamycin (5000 µg/mL) 50 mL

Kanamycin is an aminoglycoside antibiotic effective against mycoplasma, gram-positive and gram-negative bacteria. It is used in biotechnology applications to inhibit protein synthesis.

Pen-Strep-Amphotericin

This triple antibiotic solution contains penicillin (10,000 IU/mL), streptomycin (10 mg/mL), and amphotericin B (25 μg/mL).

Cellvation Cryopres Medium

Cellvation is a unique cryopreservation medium designed for optimal cell recovery and convenience. It does not contain DMSO or serum, and is scientifically engineered for Serum-Free cultures, as well as, ideally suited for all types of serum. To complete the serum-free product range, MP introduces CellVationtm, a unique cryopreservation medium which does not contain either DMSO or serum. This cell freezing medium offers the following benefits: – No presence of serum during freeze/thaw-cycles of cells adapted to serum-free growth – Absence of cytotoxicity or morphological differentiation associated with DMSO – Optimal recovery and faster cell attachment to substrate – Ready to use CellVation has a proven track record of successful freeze/thaw cycles of over 30 cell types, including primary and finite life cells as well as suspension and anchorage-dependent cell lines.

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EDTA Disodium Salt, 0.2% Solution, in Phosphate Buffered Saline without Calcium and Magnesium

EDTA Disodium Salt, 0.2% Solution, in Phosphate Buffered Saline without Calcium and Magnesium

Epidermal Growth Factor 95%

Epidermal growth factor or EGF is a growth factor isolated from mouse submaxillary glands that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells.

Hemoglobin

Hemoglobin is the major component of erythrocytes, and is responsible for the red color. Its normal concentration in erythrocytes is approximately 34%. It is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen to tissues and carries carbon dioxide away from the tissues. Anomalous globins in which various amino acids have been substitued with others, or in which certain amino acids are missing entirely from the normal sequence, comprise 153 abnormal hemoglobin configurations. Sickle cell anemia is one such configuration. In sickle cell hemoglobin, a valine residue has replaced the glutamic acid residue at position 6 in the b-chain of normal hemoglobin; the a-chain is normal.

HEPES Buffer Solution (1M)

HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions. Buffer strength for cell culture applications is usually in the range of 10 to 25 mM. Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks' and Earle's solutions are used.

HEPES Buffer

Powdered
Cell Culture Grade
Storage temperature: 15-30°C

Human Type AB Serum (HCV, HIV, HbsAG Free), Heat Inactivated

This serum is collected from Type AB human blood.

Human Type AB Serum From Male Donors

This serum is collected from Type AB human blood.

Human Type AB Serum (HCV, HIV, HbsAG Free)

This serum is collected from Type AB human blood.

Human Serum, Pooled (HCV, HIV, HBsAG Free)

This is pooled human serum obtained from normal, clotted blood.

Hybridoma Cell Growth Supplement

(HCGS)
From Cultured Human Endothelial Cells
HCGS will significantly improve the hybridoma cell cloning efficiency while eliminating feeder cell need.
HCGS may be used in dilutions of 1:5 to 1:10 (v/v). We recommend HCGS be thawed and sterile filtered (0.22 or 0.45 membrane) immediately prior to use.

Interleukin-2, Human, Natural

Human Interleukin-2 (IL-2), or Human T-cell growth factor is a glycoprotein which promotes in-vitro proliferation and long-term growth of T-cell lymphokines. Additionaly, IL-2 stimulates production of cytotoxic lymphocytes, natural killer cells, and lymphokine-activated killer cells.

Lamb Serum

This serum is derived from whole blood of healthy young lambs.

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200 mM L-Glutamine (Frozen)

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

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Phenol Red, 0.5% Solution

Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.

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Neutral Red Solution (100 mL)

1:300 Solution. Used as a red nuclear counterstain in various cytochemical procedures.

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Trypan Blue, 0.4% Solution In PBS

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

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Water For Cell Culture

For use in the preparation of cell culture media, and cell suspension and washing solutions.

}{3 AT (POWDER) UN3077-III (9)

Bulk powder, easily weighed to make custom batch sizes.

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}{ 5-FOA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Soy Hydrolysate Enzymatic

Soy Hydrolysate Enzymatic

Yeast Hydrolysate Enzymatic

Yeast Hydrolysate Enzymatic

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrochloride

Poly-L-Lysine Hydrochloride

Yeast Lytic Enzyme

Yeast Lytic Enzyme

Yeast Lytic Enzyme

Yeast Lytic Enzyme

Adult Bovine Serum

Adult Bovine Serum

Paraformaldehyde Solution

Paraformaldehyde Solution

Donor Horse Serum

This serum is processed from whole blood collected from a controlled herd of young horses. Each animal is routinely screened for Equine Infectious Anemia (EIA). Donor Horse Serum is suitable for use in diagnostic assays, as a supplement in mycoplasma growth and assay media and for culturing hematopoietic stem cells and neuronal cells.

Rabbit Serum

This serum is derived from the blood of healthy adult animals. Rabbit serum is used to support cultures of Borrelia burgdorferi, the causative agent of Lyme disease.

X-GAL (POWDER) (m)

Bulk powder, easily weighed to make custom batch sizes.

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Pen-Strep

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin.

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Amphotericin-B (250 µg/mL) 20 mL

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols, primarily ergosterols, of fungal and bacterial cell membranes. It is an antibiotic solution effective against yeast and other fungi. Amphotericin B may be toxic to some insect cell types. Amphotericin B, is an antimycotic.

G418 Sulfate Solution

G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast,higher plants, mammalian cells, protozoans and helminths. The resistance genes are located on transposons Tn601(903) and Tn5 and are bacterial in derivation, but they can be expressed in eukaryotic cells. By introducing these genes into cells, resistance to G418 Sulfate is conferred. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.

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Gentamicin Sul,10 mg/mL

Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1 (C21H43N5O7), C1a (C19H39N5O), and C2 (C20H41N5O7). Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base. Gentamicin sulfate is a broad spectrum antibiotic. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.

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Gentamicin Sul,50 mg/mL

Gentamicin Reagent Solution is effective against gram-positive and gram-negative bacteria, as well as mycoplasma.

Human Serum, Pooled

This serum is obtained from pooled, normal clotted human blood.

Goat Serum

This serum is produced from whole blood collected from healthy adult animals.

Goat Serum, Heat Inactivated

Goat Serum is used as a supplement to cell culture media. Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Be certain to maintain Good Cell Culture Practice, and maintain sterility of cultures that require media supplementation.

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Phosphate Buffered Saline Tablets (PBS)

These balanced salt tablets are specifically formulated for Immunoassay procedures.

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PENICILLIN-STREPTOMYCIN 100ML

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 10,000 units/mL of penicillin and 10,000 ug/mL of streptomycin.

Penicillin-Streptomycin (20,000 IU Pen/mL, 20,000 µg Strep/mL)

Antibiotics used to prevent bacterial contamination of cell cultures.

Bovine Brain Extract

Filtered Bovine Brain Extract used for various endothelial cell type culture.

Bovine Pituitary Extract

Filtered Bovine Pituitary Extract for cell culture.

CellMaxx™ Fetal Bovine Serum, New Zealand Origin

The growth supplement of choice in cell culture due to its high level of nutrients.