Cell Culture Reagents
Human Type Ab Serum (HCV, HIV, HbsAG Free), Heat Inactivated
This serum is collected from Type AB human blood.
The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin.
Amphotericin-B (250 µg/mL) 20 mL
Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols, primarily ergosterols, of fungal and bacterial cell membranes. It is an antibiotic solution effective against yeast and other fungi. Amphotericin B may be toxic to some insect cell types. Amphotericin B, is an antimycotic.
G418 Sulfate Solution
G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast,higher plants, mammalian cells, protozoans and helminths. The resistance genes are located on transposons Tn601(903) and Tn5 and are bacterial in derivation, but they can be expressed in eukaryotic cells. By introducing these genes into cells, resistance to G418 Sulfate is conferred. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.
Gentamicin Sul,10 mg/mL
Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1 (C21H43N5O7), C1a (C19H39N5O), and C2 (C20H41N5O7). Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base. Gentamicin sulfate is a broad spectrum antibiotic. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.
Goat Serum, Heat Inactivated
Goat Serum is used as a supplement to cell culture media. Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Be certain to maintain Good Cell Culture Practice, and maintain sterility of cultures that require media supplementation.
Endothelial Cell Growth Supplement
From Bovine Brain
Lyophilized powder with streptomycin sulfate.
ECGS is a broad culture medium growth supplement containing several growth factors which supports the growth of mammalian vascular endothelial cells and can be used in replacement of cell feeder layers in support of hybridoma clonal growth. It is isolated from bovine brain by a modification of the method of Maciag.
Ref.: Maciag, et al., Proc. Nat'l. Acad. Sci. USA, 76: 5674-5678 (1979).
Trypan Blue, 0.4% Solution In PBS
Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.