Cell Culture Reagents
Human Type Ab Serum (HCV, HIV, HbsAG Free), Heat Inactivated
This serum is collected from Type AB human blood.
Trypan Blue, 0.4% Solution In PBS
Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
Endothelial Cell Growth Supplement
From Bovine Hypothalamus
Lyophilized from sodium chloride buffer containing streptomycin sulfate
Activity: promotes the growth of Fetal Bovine Heart Endothelial Cells (FBHEC) in 10% newborn calf serum when reconstituted at a concentration of 50-150 μg/ml serum-free medium.
It contains several growth factors (e.g. FGF, ECGF-α) and supports the growth of vascular endothelial cells from human and other mammalian species. Also, it may be used for the cloning and proliferation of hybridomas and human keratinocytes.
Each lot is sterile filtered and tested for bacteria, fungi, and mycoplasma contamination. Some cells may require supplementation with 1-100 μg/ml heparin.
Epidermal Growth Factor 95%
Epidermal growth factor or EGF is a growth factor isolated from mouse submaxillary glands that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells.
Hemoglobin is the major component of erythrocytes, and is responsible for the red color. Its normal concentration in erythrocytes is approximately 34%. It is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen to tissues and carries carbon dioxide away from the tissues. Anomalous globins in which various amino acids have been substitued with others, or in which certain amino acids are missing entirely from the normal sequence, comprise 153 abnormal hemoglobin configurations. Sickle cell anemia is one such configuration. In sickle cell hemoglobin, a valine residue has replaced the glutamic acid residue at position 6 in the b-chain of normal hemoglobin; the a-chain is normal.