Cell Culture Reagents

55 Results Found

Lamb Serum

This serum is derived from whole blood of healthy young lambs.

Human Serum, Pooled (HCV, HIV, HBsAG Free)

This is pooled human serum obtained from normal, clotted blood.

Human Type Ab Serum (HCV, HIV, HbsAG Free)

This serum is collected from Type AB human blood.

Human Type Ab Serum From Male Donors

This serum is collected from Type AB human blood.

Human Type Ab Serum (HCV, HIV, HbsAG Free), Heat Inactivated

This serum is collected from Type AB human blood.

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Pen-Strep

The antibiotics penicillin and streptomycin are used to prevent bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria. This solution contains 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin.

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Amphotericin-B (250 µg/mL) 20 mL

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols, primarily ergosterols, of fungal and bacterial cell membranes. It is an antibiotic solution effective against yeast and other fungi. Amphotericin B may be toxic to some insect cell types. Amphotericin B, is an antimycotic.

G418 Sulfate Solution

G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast,higher plants, mammalian cells, protozoans and helminths. The resistance genes are located on transposons Tn601(903) and Tn5 and are bacterial in derivation, but they can be expressed in eukaryotic cells. By introducing these genes into cells, resistance to G418 Sulfate is conferred. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.

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Gentamicin Sul,10 mg/mL

Gentamicin is an aminoglycoside antibiotic complex produced by fermentation of Micromonospora purpurea or M. echinospora. It is a mixture of 3 major components designated as C1 (C21H43N5O7), C1a (C19H39N5O), and C2 (C20H41N5O7). Gentamicin is used as the sulfate salt. Each component consists of five basic nitrogens and requires five equivalents of sulfuric acid per mole of gentamicin base. Gentamicin sulfate is a broad spectrum antibiotic. It inhibits the growth of a wide variety of Gram-positive and Gram-negative microorganisms, including strains resistant to tetracycline, chloramphenicol, kanamycin, and colistin, particularly strains of Pseudomonas, Proteus, Staphylococcus, and Streptococcus. It is effective against mycoplasma as well.

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Gentamicin Sul,50 mg/mL

Gentamicin Reagent Solution is effective against gram-positive and gram-negative bacteria, as well as mycoplasma.

Human Serum, Pooled

This serum is obtained from pooled, normal clotted human blood.

Goat Serum

This serum is produced from whole blood collected from healthy adult animals.

Goat Serum, Heat Inactivated

Goat Serum is used as a supplement to cell culture media. Goat Serum provides a broad spectrum of macromolecules, carrier proteins for lipoid substances and trace elements, attachment and spreading factors, low molecular weight nutrients, and hormones and growth factors that promote cell growth and health. Be certain to maintain Good Cell Culture Practice, and maintain sterility of cultures that require media supplementation.

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Phosphate Buffered Saline Tablets (PBS)

These balanced salt tablets are specifically formulated for Immunoassay procedures.

Endothelial Cell Growth Supplement

(ECGS)
From Bovine Brain
Lyophilized powder with streptomycin sulfate.
ECGS is a broad culture medium growth supplement containing several growth factors which supports the growth of mammalian vascular endothelial cells and can be used in replacement of cell feeder layers in support of hybridoma clonal growth. It is isolated from bovine brain by a modification of the method of Maciag.
Ref.: Maciag, et al., Proc. Nat'l. Acad. Sci. USA, 76: 5674-5678 (1979).

Neutral Red Solution (100 mL)

1:300 Solution. Used as a red nuclear counterstain in various cytochemical procedures.

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Trypan Blue, 0.4% Solution In PBS

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

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Water For Cell Culture

For use in the preparation of cell culture media, and cell suspension and washing solutions.

}{3 AT (POWDER) UN3077-III (9)

Bulk powder, easily weighed to make custom batch sizes.

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}{ 5-FOA (POWDER)

Bulk powder, easily weighed to make custom batch sizes.