Cell Culture Reagents
Human Type Ab Serum (HCV, HIV, HbsAG Free), Heat Inactivated
This serum is collected from Type AB human blood.
Cellvation Cryopres Medium
Cellvation is a unique cryopreservation medium designed for optimal cell recovery and convenience. It does not contain DMSO or serum, and is scientifically engineered for Serum-Free cultures, as well as, ideally suited for all types of serum. To complete the serum-free product range, MP introduces CellVationtm, a unique cryopreservation medium which does not contain either DMSO or serum. This cell freezing medium offers the following benefits: – No presence of serum during freeze/thaw-cycles of cells adapted to serum-free growth – Absence of cytotoxicity or morphological differentiation associated with DMSO – Optimal recovery and faster cell attachment to substrate – Ready to use CellVation has a proven track record of successful freeze/thaw cycles of over 30 cell types, including primary and finite life cells as well as suspension and anchorage-dependent cell lines.
Endothelial Cell Growth Supplement
From Bovine Hypothalamus
Lyophilized from sodium chloride buffer containing streptomycin sulfate
Activity: promotes the growth of Fetal Bovine Heart Endothelial Cells (FBHEC) in 10% newborn calf serum when reconstituted at a concentration of 50-150 μg/ml serum-free medium.
It contains several growth factors (e.g. FGF, ECGF-α) and supports the growth of vascular endothelial cells from human and other mammalian species. Also, it may be used for the cloning and proliferation of hybridomas and human keratinocytes.
Each lot is sterile filtered and tested for bacteria, fungi, and mycoplasma contamination. Some cells may require supplementation with 1-100 μg/ml heparin.
Epidermal Growth Factor 95%
Epidermal growth factor or EGF is a growth factor isolated from mouse submaxillary glands that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells.
Trypan Blue, 0.4% Solution In PBS
Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
Hemoglobin is the major component of erythrocytes, and is responsible for the red color. Its normal concentration in erythrocytes is approximately 34%. It is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen to tissues and carries carbon dioxide away from the tissues. Anomalous globins in which various amino acids have been substitued with others, or in which certain amino acids are missing entirely from the normal sequence, comprise 153 abnormal hemoglobin configurations. Sickle cell anemia is one such configuration. In sickle cell hemoglobin, a valine residue has replaced the glutamic acid residue at position 6 in the b-chain of normal hemoglobin; the a-chain is normal.
1M HEPES Buffer
HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a general purpose zwitterionic organic chemical buffering agent which does not bind magnesium, calcium, manganese(II) or copper (II) ions. Buffer strength for cell culture applications is usually in the range of 10 to 25 mM. Studies have indicated that 20 mM HEPES is the most satisfactory concentration of the buffer when both Hanks' and Earle's solutions are used.