Tris
Application Notes
Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Electrophoresis
| SKU | 02103133-CF |
| Alternate Names | 2-Amino-2-(hydroxymethyl)-1,3-propanediol; THAM; Tris base; Trometamol; Tromethamine; Tris-[hydroxymethyl]amino-methane |
| Applications | Electrophoresis |
| Application Notes | Tris is used in pH control in vitro and in vivo for body fluids and in buffering systems for electrophoresis applications.Tris is used in assays used to characterize the activity and kinetics of the enzymes that catalyze SUMOylation of Small ubiquitin-like proteins (SUMO) and SUMO-dependent protein-protein interactions |
| Base Catalog Number | 103133 |
| Beilstein Registry Number | 741883 |
| Boiling Point | 219-220 °C at 10 mm Hg |
| CAS # | 77-86-1 |
| EC Number | 201-064-4 |
| Grade | Ultra Pure |
| Hazard Statements | H315-H319-H335 |
| Melting Point | 165.0 - 175.0°C |
| Molecular Formula | NH2C(CH2OH)3 |
| Molecular Weight | 121.136 g/mol |
| Personal Protective Equipment | Dust mask, Eyeshields, Gloves. |
| pH | 10.0 - 11.5 (5% aq soln) |
| pKa | 8.1 at 25 °C (Lit.) |
| Purity | ≥99.9% |
| RTECS Number | TY2900000 |
| Safety Symbol | GHS07 |
| Solubility | Soluble in water (550 mg/ml), ethylene glycol (79.1 mg/ml), methanol (26 mg/ml), anhydrous ethanol (14.6 mg/ml), 95% ethanol (22.0 mg/ml), DMF (14 mg/ml), acetone (2 mg/ml), ethyl acetate (0.5 mg/ml), olive oil (0.4 mg/ml), and chloroform (0.05 mg/ml) |
| Sterilization of Solutions | Tris solutions can be autoclaved (121°C, 15 psi, 15 minutes) or sterile filtered. |
| Storage | Room Temperature (15-30 °C) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Pressure | 2.2X10-5 mm Hg at 25 °C (est) |