Product Description
Methotrexate
Application Notes
Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites. Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Anti-tumor studies.
Specifications
| SKU | 0210229925 |
| Alternate Names | Amethopterin; N-[4-[[(2,4-Diamino-6-pteridinyl) methyl] methylamino] benzoyl]-L- glutamic acid; 4-amino-N10-methylpteroylglutamic acid; 4-amino-10-methylfolic acid; methylaminopterin; MTX; CL-14377; Emtexate; Ledertrexate; A-Methopterin; Rheumatrex |
| Application Notes | Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also effective in treatment of pyrimethamine-resistant Plasmodium vivax malaria parasites. Potent inhibitor of dihydrofolate reductase and agent for antitumor studies. Use to inhibit dihydrofolate reductase in DHFR-based protein expression systems. Also shows immunosuppressive effects in, e.g., rheumatoid arthritis. |
| Base Catalog Number | 10229925 |
| Biochemical Physiological Actions | Methotrexate is an allosteric inhibititor of dihydrofolate reductase (DHFR), the enzyme that catalyzes the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrolfolate is required for purine and pyrimidine synthesis, methotrexate treatment results in the inhibition of DNA and RNA synthesis. |
| CAS # | 59-05-2 |
| EC Number | 200-413-8 |
| Format | Powder |
| Grade | USP |
| Melting Point | 365 to 399° F (decomposes) (NTP, 1992) |
| Molecular Formula | C20H22N8O5 |
| Molecular Weight | 454.447 g/mol |
| Optical Rotation | +19° to +24° |
| Pack Size | 25 mg |
| Purity | ≥98% |
| RTECS Number | MA1225000 |
| Solubility | less than 1 mg/mL at 66° F (NTP, 1992) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Pressure | 2.1X10-19 mm Hg at 25 deg C /Estimated/ |
Documents
Certificate of Analysis (COA)
Datasheet
Datasheet
Brochures
Brochures