Bovine Transferrin is an iron-depleted product supplied as a heat-treated, lyophilised powder.
Application Notes
Bovine Transferrin (APO) is frequently used as a nutrient in cell and microbial culture and as a media supplement in bio-pharmaceutical production. It is a useful transport factor in cell culture work to convey essential metabolites to the cells. Important factor when culturing cells in a defined medium.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Nutrient in cell and microbial culture | media supplement in bio-pharmaceutical production
SKU | 02152334-CF |
Application Notes | Bovine Transferrin (APO) is frequently used as a nutrient in cell and microbial culture and as a media supplement in bio-pharmaceutical production. It is a useful transport factor in cell culture work to convey essential metabolites to the cells. Important factor when culturing cells in a defined medium. |
Background_information | All cells require iron for the proper uptake of oxygen from their environment. Transferrin, a protein isolated from serum, causes the uptake and transport of iron from culture medium to the cells. This allows proper oxygen uptake and also stimulates growth-related enzyme activity. Iron-saturated transferrin (holo transferrin) is continually recycled after releasing iron and becomes iron-deficient transferrin (apo-transferrin). Choosing which form to use depends upon the culture conditions. In media such as Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F12 (DMEM/F12) that contain high levels of iron salt, using apo-transferrin is preferred. |
Base Catalog Number | 152334 |
CAS # | 11096-37-0 |
EC Number | 234-318-8 |
Format | Lyophilized powder |
Grade | Cell Culture Grade |
Host | Bovine |
Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
Preparation Method | Bovine Transferrin is chromatographically purified from New Zealand-sourced bovine plasma in an ISO quality system assuring complete traceability and consistent high quality. |
Protein or Enzyme Type | Transferrin |
Purity | ≥95% (w/w total protein) |
Solubility | Soluble in water (3% w/v): Dissolves in ≤20 min. |
Source | Bovine Plasma |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |