An anionic detergent typically used to solubilize and denature proteins for electrophoresis. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material.12 Most proteins bind SDS in a ratio of 1.4 grams SDS to 1 gram protein. The charges intrinsic to the protein become insignificant compared to the overall negative charge provided by the bound SDS. The charge-to-mass ratio is essentially the same for each protein and will migrate in the gel based only on protein size.
Application Notes
SDS is used to solubilize and denature protein for Protein Electrophoresis & Blotting. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. Surfactant used for mild, efficient, and greener dethioacetalization.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Denaturing proteins
SKU | 02198957-CF |
Alternate Names | Sodium Lauryl Sulfate; Sulfuric Acid, Monododecyl Ester, Sodium Salt; Dodecyl Sulfate, sodium salt; SLS; SDS; Neutrazyme; Lauryl Sulfate Sodium Salt; SDS Running Buffer |
Application Notes | SDS is used to solubilize and denature protein for Protein Electrophoresis & Blotting. SDS has also been used in large-scale phenol extraction of RNA to promote the dissociation of protein from nucleic acids when extracting from biological material. Surfactant used for mild, efficient, and greener dethioacetalization. |
Base Catalog Number | 198957 |
Beilstein Registry Number | 3599286 |
CAS # | 151-21-3 |
Critical Micelle Concentration | 8.27 mM |
Density | greater than 1.1 at 68° F (USCG, 1999) |
EC Number | 205-788-1 |
Format | Liquid |
Formulation Details | 20% SDS; 80% water |
Hazard Statements | H315-H318-H335 |
Melting Point | 399 to 405° F (NTP, 1992) |
Personal Protective Equipment | Eyeshields, Faceshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge,respirator filter |
RTECS Number | WT1050000 |
Safety Symbol | GHS05, GHS07 |
Solubility | less than 1 mg/mL at 66° F (NTP, 1992) |
Typical Working Concentration | >10 mg detergent per mg of protein. (Lit.) |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |