An aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.
Application Notes
Hygromycin B is used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
In gene transfer experiments as a selection agent.
| SKU | 0219417083 |
| Alternate Names | O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- (1->2-3)-O-b-D- talopyranosyl- (1->5)- 2-deoxy- N3-methyl-D-streptamine |
| Application Notes | Hygromycin B is used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. |
| Base Catalog Number | 194170 |
| Biochemical Physiological Actions | Blocks polypeptide synthesis and inhibits elongation. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template. |
| CAS # | 31282-04-9 |
| EC Number | 250-545-5 |
| Hazard Statements | H300 +H310 + H330-H318-H334 |
| Molecular Formula | C20H37N3O13 |
| Molecular Weight | 527.50 |
| Pack Size | 250 mg |
| pKa | 7.1 |
| Purity | 95% |
| RTECS Number | WK2130000 |
| Safety Symbol | GHS05, GHS06, GHS08 |
| Source | Streptomyces hygroscopicus |
| Specific Activity | >1000 u/mg |
| Typical Working Concentration | When using this product for mammalian cell selection the working concentration is generally between 50 mg/mL and 1 mg/mL, but at times may go as high as 1 mg/mL. The appropriate concentration for use must be determined empirically for each cell type. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [194170] lot ID: M4168](https://www.mpbio.com/static/version1744790984/frontend/Mpbio/default/en_US/images/document.png "MP COA [194170] lot ID: M4168")