Elastase, ≥50 U/mg

Name: Elastase, ≥50 U/mg
SKU: 02100617-CF

Key features and details

Elastase

SKU: 02100617-CF

Synonyms

Elastase from hog pancreas; Pancreatopeptidase E

CAS Number:

2594837

EC Number:

254-453-6

MDL Number:

MFCD00130998

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Product Description

Elastase

Application Notes

Elastase was used to study human emphysema by induction of emphysema in rodents. It has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Investigate the purification and partial characterization of the pancreatic proteolytic enzymes

Specifications

SKU	02100617-CF
Alternate Names	Elastase from hog pancreas; Pancreatopeptidase E
Application Notes	Elastase was used to study human emphysema by induction of emphysema in rodents. It has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase. It hydrolyzes peptide bonds, especially those adjacent to neutral amino acids.
Base Catalog Number	100617
Biochemical Physiological Actions	Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.
CAS #	2594837
EC Number	254-453-6
Format	Solution
Formulation Details	Aqueous suspension with 0.01% sodium azide as preservative
Grade	2× Crystallized
Hazard Statements	H315-H319-H335
Personal Protective Equipment	Dust mask, Eyeshields, Faceshields, Gloves
Preparation Method	Elastase is prepared from porcine pancreas. It is lyophilized and water soluble. Elastase is chromatographically prepared by the method of Narayanan and Anwar. In the method, 2 times crystalline elastase is adsorbed on a column of DEAE-Sephadex A50 to separate elastase and non-specific protein components. The elastase component is further purified by chromatography on a column of CM-Cellulose using a sodium chloride gradient to elute the elastase. The latter is dialyzed until chloride-free and then lyophilized. During its preparation the elastase is held below a pH of 5.5 for greater than 24 hours. Two times crystallized from the euglobin fraction of porcine pancreas by the method of Lewis et al. Does not contain trypsin or chymotrypsin.
RTECS Number	JX7069000
Safety Symbol	GHS07
Source	Porcine pancreas
Specific Activity	Activity ≥50 u/mg protein
Unit Definition	One unit will solublize 1 mg of elastin in 20 minutes at 37 °C, pH 8.8.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.