SPINeasy Plasmid Miniprep Kit is a silica-membrane spin-column kit that enables up to 20 µg of plasmid DNA to be purified from 1 – 5 mL of transformed bacterial culture. High purity plasmid DNA can be obtained within 25 minutes through a simple purification process and is immediately ready for routine molecular biology laboratory applications.
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Figure 1: Agarose gel electrophoresis of plasmid DNA extracted with the SPINeasy Plasmid Miniprep Kit. M: DNA marker; Lane 1: pWPI plasmid; Lane 2: pWPI plasmid linearized by restriction digest; Lane 3: pcDNA6 plasmid; Lane 4: pcDNA6 plasmid linearized by restriction digest. |
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Application Notes
The SPINeasy Plasmid Miniprep Kit quickly and efficiently purifies up to 20 µg plasmid DNA from 1-5 mL of transformed bacterial culture within 25 minutes through a simple purification process.
Usage Statement
Research Use Only (RUO).
Key Applications
In-vitro transcription, cloning, NGS, PCR, qPCR, restriction digestion, transformation, Genomics, Molecular Biology
| SKU | 116534050 |
| Alternate Names | SPINeasy Plasmid Extraction Kit; Plasmid Miniprep Kit; Plasmid DNA Purification |
| Application Notes | The SPINeasy Plasmid Miniprep Kit quickly and efficiently purifies up to 20 µg plasmid DNA from 1-5 mL of transformed bacterial culture within 25 minutes through a simple purification process. |
| Base Catalog Number | 65340 |
| Product Line | SPINeasy |
| Components | 20 mL Resuspension Buffer SN1, 20 mL Alkaline Lysis Buffer SN2, 30 mL Neutralization Buffer SN3, 21 mL Wash Buffer NW1, 6 mL Wash Buffer NW2, 10 mL Elution Buffer SN, 200 µL RNase A, 50x Column SN with collection tubes |
| Hazard Statements | Hazard Class 8 |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | Store at Room Temperature (15-30℃), Dry environment |
| Usage Statement | Research Use Only (RUO). |
