Mycoplasma Hoechst Stain Kit is designed for rapid in situ detection of mycoplasma, and other prokaryotic organisms, in cell cultures in less than two hours. This procedure is based on the Hoechst Stain method cited by the Tissue Culture Association (TCA Procedure No, 75361.) Several DNA fluorochromes such as DAPI, quinacrine mustard and quinacrine dihydrochloride have been used in the same technique but none of the fluorochromes perform as well as the Hoechst stain with respect to fluorescent effect, slow quenching and minimum background fluorescence. The Hoechst Stain method selectively binds minor grooves of the DNA, which allows for efficient staining of the nuclei.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Detection of mycoplasma
|Base Catalog Number||3030000|
|Personal Protective Equipment||Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Handle with gloves skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Specificity||This stain is highly specific for DNA. It is thought to occupy one of the grooves of the DNA double helix; binding by intercalation. The stain will cause cellular nuclei to fluoresce; however; the other major DNA cytoplasmic components - mitochondria - will not. All prokaryotic organisms (Mycoplasmas; bacteria; yeast; fungi) will give off fluorescence. The identification of the contaminant must be made by noting size; morphology and relationship to the cell.|
|Typical Working Concentration||The Hoechst Stain should give maximum fluorescent intensity when diluted 1:10 with Hanks Balanced Salt Solution, pH 7.0, achieving a final concentration at 0.05 µg/mL.|
|Usage Statement||Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.|