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Anti-rabbit IgG (whole molecule) affinity-purified goat antibody, fluorescein-conjugated, 2 mg

Anti-rabbit IgG (whole molecule) affinity-purified goat antibody, fluorescein-conjugated, 2 mg

₹29,257.50

Key features and details

Fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.

SKU: 0855662

MDL Number:
MFCD00162786
Packaging size:
2 mg
Product Description

Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.

Application Notes

Fluorescein-conjugated goat affinity purified antibody to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855662
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-conjugated goat affinity purified antibody to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
Base Catalog Number 55662
Concentration Total Protein: 1.0 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate FITC
Formulation Details Purified
Host Goat
Immunogen Purified rabbit IgG
Pack Size 2 mg
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 μm filter, and vialed.
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Rabbit (Whole Molecule)
Specificity Product shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Typical Working Concentration Anti-Rabbit IgG (whole molecule)-FITC antibody may be used for immunofluorescent staining of human peripheral blood lymphocytes at a working dilution of 1:160. A minimum working dilution of 1:160 may be used on formalin-fixed, paraffin-embedded human tonsil sections and Anti-Human IgG as the primary antibody. This antibody was used for immunefluorescence of Alfalfa root and leaf sections stained with anti-Medicago sativa GSK-3-like kinase primary antibody. For immunostaining of human testis cryosections, a dilution of 1:200 was used with N-cadherin primary antibody.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificate of Analysis

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Certificate of Analysis
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Certificate of Analysis
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Datasheet

Datasheet