Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to rabbit complement C3 and buffer salts.
This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Immunoassays | Immunofluorescence assays (IFA) | Immunoblot
|Antibody Type||Polyclonal Antibody|
|Application Notes||This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.|
|Base Catalog Number||55654|
|Concentration||Total Protein: 23.0 mg/ml F/P Ratio: 5.4 mol/mol Antibody Protein: 7 mg/ml|
|Pack Size||2 mL|
|Preparation Method||Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.|
|Solubility||Soluble in water|
|Specificity||The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected. This product shows reactivity to rabbit complement C3; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.|