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Anti-mouse IgG (whole molecule) affinity-purified a goat ntibody, fluorescein-conjugated, 2 mg

Anti-mouse IgG (whole molecule) affinity-purified a goat ntibody, fluorescein-conjugated, 2 mg

₹27,337.50

Key features and details

Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

SKU: 0855514

MDL Number:
MFCD00162642
Packaging size:
2 mg
Product Description

Product is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

Application Notes

Fluorescein-Conjugated Goat Affinity Purified Antibody to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855514
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat Affinity Purified Antibody to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. It is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
Base Catalog Number 55514
Concentration Total Protein: 0.8 mg/mlF/P Ratio: 4.1 mol/molActive Antibody: 0.8 mg/ml
Conjugate FITC
Formulation Details Purified
Host Goat
Immunogen Purified mouse IgG
Pack Size 2 mg
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 μm filter, and vialed.
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Mouse (Whole Molecule)
Specificity This product shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Typical Working Concentration Anti-Mouse IgG (whole molecule)-FITC antibody is suitable for use in immunohistochemistry (1:200) and immunofluorescence (1:100).
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
French (France)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)