Cart
 
Inquire

Anti-human IgA (alpha chain) goat IgG fraction, fluorescein-conjugated, 2 mL

Anti-human IgA (alpha chain) goat IgG fraction, fluorescein-conjugated, 2 mL

₹39,360.00

Key features and details

Lyophilized fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human IgA (α chain) and buffer salts.

SKU: 0855077

MDL Number:
MFCD00162415
Packaging size:
2 mL
Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human IgA (α chain) and buffer salts.

Application Notes

This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855077
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Base Catalog Number 55077
Concentration Total Protein: 47.8 mg/mL; Antibody Protein: 6 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Purity Purified
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human (Alpha Chain)
Specificity The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected. This product shows reactivity to human IgA; cross-reactivity to other species may exist. Antibody activity to IgG; IgM; or non-immunoglobulin serum proteins is not present.
Sterility Filtered through a .22 µm filter
Typical Working Concentration Suggested working dilution: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (Australia)
Material Safety Data Sheet
English (Canada)
Material Safety Data Sheet
French (Canada)
Material Safety Data Sheet
Chinese (China)
Material Safety Data Sheet
German (Germany)
Material Safety Data Sheet
Spanish (Spain)
Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
Finnish (Finland)
Material Safety Data Sheet
French (France)
Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
Japanese (Japan)
Material Safety Data Sheet
Spanish (Mexico)
Material Safety Data Sheet
Norvegian (Norway)
Material Safety Data Sheet
Portuguese (Portugal)
Material Safety Data Sheet
Swedish (Sweden)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)

Brochures

Brochures
English (United States)
Brochures
English (United States)