Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated rabbit IgG fraction to chicken IgG (whole molecule) and buffer salts.
This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
|Antibody Type||Polyclonal Antibody|
|Application Notes||This product is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining|
|Base Catalog Number||55307|
|Concentration||Total Protein: 4.3 mg/ml F/P Ratio: 4.3 mol/mol Antibody Protein: 2 mg/ml|
|Pack Size||2 mL|
|Solubility||Soluble in water|
|Species Reactivity||Chicken (Whole Molecule)|
|Specificity||The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected. This product shows reactivity to chicken IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.|