Anti-rabbit IgG (whole molecule) affinity-purified goat F(Ab')2 fragment, peroxidase-conjugated, 1 mL
Peroxidase-conjugated goat affinity purified F(ab')2 fragment to Rabbit IgG (Whole Molecule) is horseradish peroxidase (HRP)-conjugated goat affinity purified F(ab')2 fragments to rabbit IgG (whole molecule) and buffer salts.
Application Notes
Peroxidase-conjugated goat affinity purified F(AB')2 fragment to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunohistochemistry | Immunoblotting |ELISA
| SKU | 0855693 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Peroxidase-conjugated goat affinity purified F(AB')2 fragment to Rabbit IgG (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Use of affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. Note F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. |
| Base Catalog Number | 55693 |
| Concentration | Total Protein: 1.0 mg/mL; Titer: 1:20;000 |
| Conjugate | HRP |
| Formulation Details | Purified |
| Host | Goat |
| Immunogen | Purified rabbit IgG |
| Pack Size | 1 mL |
| Preparation Method | Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 1% BSA, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. No preservative is added. |
| Purity | Affinity Purified |
| Solubility | Soluble in water |
| Species Reactivity | Rabbit (Whole Molecule) |
| Specificity | Product shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present. |
| Typical Working Concentration | Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution. Peroxidase-conjugated goat anti-rabbit IgG antibody was used for wester blot analysis of yeast lysates following protein carbonylation assays at a dilution of 1:5000. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [55693] lot ID: 01009](https://www.mpbio.com/static/version20210119154001Z/frontend/Mpbio/default/en_US/images/pdf.png "MP COA [55693] lot ID: 01009")