Sample Prep Solutions

Learn more about the complete FastPrep® sample prep solution.

Lysing Matrix Tubes

Many Optimized FastPrep Matrices to Lyse Even your Toughest Samples.

See All Matrix Options
FastPrep Insturments

The Most Advanced Sample Preparation Systems Available!

FastPrep Adapters

Ergonomic design ensures easy loading and secure homogenization.

DNA and RNA from Some of Nature's Toughest Samples
Some animal and human tissues are the toughest samples to isolate high-quality DNA or RNA. Hard Samples like bone and tumors, and more elastic samples like skin, can be difficult to lyse with other homogenization systems, but the FastDNA™ Spin Kit, FastDNA™ Spin Kit for Plant and Animal Tissue, and the FastRNA™ Pro Green Kit used in combination with the FastPrep instrument will quickly and efficiently extract PCR-ready DNA and RNA.
FastPrep-24 and Lysing Matrix for use for metabolomic studies

Dr. Jackob M. Haus, M.D. from Cleveland Clinic

Solutions for Isolation of Nucleic Acids from Tissue

The FastDNA™ Spin Kit for Animal Tissue quickly and efficiently isolates genomic DNA from a wide variety of animal sources. Designed for use with the FastPrep® Instruments from MP Biomedicals, animal tissues, are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by multidirectional, simultaneous impaction with lysing matrix particles.

FastPrep® Instruments provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods. Samples are placed into 2.0 ml tubes containing the proprietary Lysing Matrix D, which are 1.4mm ceramic spheres. While almost all samples are easily processed with Lysing Matrix D, additional lysing matrix options are available for more delicate samples or for tough, hardened samples.

Homogenization of human and animal tissues in the FastPrep® Instrument with Lysing Matrix D takes place in the presence of sample-specific Cell Lysis Solution, CLS-TC. For maximum flexibility, all buffers are provided in the kit. Following lysis, samples are centrifuged to pellet debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for digestion, electrophoresis, PCR and any other desired application.

RNA 2100 Bioanalyzer analysis of FastPrep sample. The RNA was run on an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, Calif.) using the RNA 6000 Pico LabChip kit to determine the quality of the samples. The 28S and 18S ribosomal bands show a greater than 2:1 ratio, and the calculated RNA ribosomal integrity numbers of the samples ranged from 8.4 to 8.9, verifying high-quality RNA. Shown above are the gel images for 11 RNA samples. Below are two representative electrophoretic graphs showing the RNA peaks.

Case Study, Dual Extraction of RNA and Proteins from Skin

Current translational human studies are moving in the direction of concurrent genomic and proteomic analysis using small clinical samples. Two mechanical disruption methods were tested for their efficiency in disrupting biopsy skin samples: the FastPrep System and a Polytron® 1300D homogenizer. The quantity and quality of RNA from both extraction techniques were calculated using a NanoDrop® spectrophotometer. The average yield of 1.4 µg RNA with the FastPrep System was 57% higher than with the Polytron. To verify the high-quality nature of the RNA extracted with the FastPrep system, processed samples were analyzed with the Agilent 2100 Bioanalyzer. All the samples processed with the FastPrep System had ribosomal integrity numbers of between 8.4 and 8.9, which is consistent with high-quality RNA. Protein quantitation was determined using a Coomassie-based assay using a standard containing bovine serum albumin in 8 M urea and 10 mM dithiothreitol. The average yield using the FastPrep System was 170 µg of protein, a 53% increase over the Polytron technique. The quality of extracted proteins was assessed by two dimensional gel and Western blot analysis.

Typical Tissue Sample Recommendations
Sample Name Sample Type Quantity Lysing Matrix FastPrep Speed FastPrep Time
Human Lung 50 mg D 6.0 4 x 30 sec.
Human Breast 80 mg D 6.0 2 x 30 sec
Human Kidney 50 mg D 6.0 40 sec
Human Thyroid Tumors 100 mg A 6.0 3 x 30 sec
Mouse Eye 10 mg D 6.0 4 x 30 sec.
Mouse Heart 70 mg D 6.0 4 x 30 sec.
Mouse Kidney 50 mg D 6.0 40 sec
Mouse Femur 40 mg A 6.0 4 x 30 sec.
Mouse Leg Muscle 50 mg D 6.0 40 sec
Mouse Intestine 50 mg D 6.0 40 sec
Mouse Ear 45 mg D 6.0 4 x 30 sec.
Mouse Tail 100 mg A 6.0 4 x 30 sec.
Mouse Spleen 70 mg D 6.0 40 sec
Mouse Lung 50 mg D 6.0 40 sec
Mouse Liver 50 mg D 6.0 40 sec
Mouse Brain 50 mg D 6.0 40 sec
Mouse Pancreatic cells (bHC9) 107 cells D 6.0 40 sec
FastPrep Kits & Instruments
Purification Kits SKU Free Sample (Availability)
FastDNA™ Spin Kit 116540600
FastDNA™ Spin Kit for Plant and Animal Tissue 116540800 N/A
FastRNA™ Pro Green Kit 116045050 N/A
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