Sample Prep Solutions

Learn more about the complete FastPrep® sample prep solution.

Lysing Matrix Tubes

Many Optimized FastPrep Matrices to Lyse Even your Toughest Samples.

See All Matrix Options
FastPrep Insturments

The Most Advanced Sample Preparation Systems Available!

FastPrep Adapters

Ergonomic design ensures easy loading and secure homogenization.

Bacteria are the major component of activated sludge and are responsible for the oxidation of organic matter and nutrient transformations, as well as the production of polysaccharides and other polymeric materials that aid in the flocculation of the microbial biomass. The predominant bacterial phyla are the Proteobacteria, Planctomycetes, Bacteroidetes, Firmicutes, and Actinobacteria.
The investigation of microbial population and their diversity in activated sludges are important for control and maintenance of wastewater treatment system. Moreover, the obtained information provided new insight in microbiology which helped to improve the design and performance of new generation reactors. However, conventional methods such as plate or liquid culture are inadequate for microbial diversity study because of limited knowledge on the real growth conditions of most microorganisms in their habitats and the difficulty of media development for cultivation. Today, molecular methods are used to investigate bacterial communities in activated sludge. The key success of this approach lies in the quality of nucleic acids isolated from the environmental samples.

DNA extraction from activated sludge has been performed for two decades for DNA probing, PCR amplification of specific targets, and the construction of clonal libraries. For these purposes, sample lysis needs to be complete without excessive shearing of the DNA, which can increase artefact formation during the PCR. The extracted nucleic acid must be free of contaminants that can inhibit enzymes used in subsequent molecular techniques, such as restriction endonucleases or Taq polymerase. For quantitative studies of mixed-liquor population dynamics using real-time PCR, the DNA extraction method also needs to provide uniform DNA recovery and high throughput.

The FastPrep Family product line provides a powerful molecular tool for gaining insight into microbial communities in sludge samples. FastDNA™ and FastRNA™ kits for soil used in combination with the FastPrep-24™ or FastPrep-96™ instrument will help overcome any difficulties with complete lysis of all sludge bacteria and isolation of pure DNA and RNA.

Typical Sludge Sample Recommendations
Sample Name Quantity Lysing Matrix FastPrep Speed FastPrep Time
Sludge 50 mg E 5 4 x 40 sec
FastPrep Kits & Instruments
Purification Kits SKU Free Sample (Availability)
FastDNA™ and FastRNA™ kits for soil 116540600
FastRNA™ Pro Soil-Direct Kit
FastRNA™ Pro Soil-Indirect Kit
Publications and References
Adamczyk, J., et al. (2003) The Isotope Array, a New Tool That Employs Substrate-Mediated Labeling of rRNA for Determination of Microbial Community Structure and Function. Applied And Environmental Microbiology 69, 6875-6887.
Diaz, E.E., et al. (2006) Phenotypic Properties and Microbial Diversity of Methanogenic Granules from a Full-Scale Upflow Anaerobic Sludge Bed Reactor treating Brewery Wastewater. Applied And Environmental Microbiology 72, 4942-4949.
Yu, C.P., et al. (2005) Quantitative Molecular Assay for Fingerprinting Microbial Communities of Wastewater and Estrogen-Degrading Consortia. Applied And Environmental Microbiology 71, 1433-1444.
Dionisi, H.M., et al. (2003) power Analysis for Real-Time PCR Quantification of Genes in Activated Sludge and Analysis of the Variability Introduced by DNA Extraction. Applied And Environmental Microbiology 69, 6597-6604.