Sample Prep Solutions

Learn more about the complete FastPrep® sample prep solution.

Lysing Matrix Tubes

Many Optimized FastPrep Matrices to Lyse Even your Toughest Samples.

See All Matrix Options
FastPrep Insturments

The Most Advanced Sample Preparation Systems Available!

FastPrep Adapters

Ergonomic design ensures easy loading and secure homogenization.

One gram of sediment contains more than 10e10 bacteria and sediment has been estimated to contain up to 12000 different genomes, which is the highest for any environment. Most of the marine bacteria and archae have been shown to be difficult to culture, or have not been yet cultured. Thus, the study of, and access to, the collective genomes of this "hidden" diversity requires the application of molecular techniques. One new molecular tool which has become very powerful is metagenomics.
The construction of metagenomic libraries and other DNA-based metagenomic projects are initiated by isolation of high-quality DNA that is suitable for cloning and covers the microbial diversity present in the original sample. DNA isolation, especially from extreme environments, is still a technological challenge. Reasons for this include the reluctance of many microorganisms present in these samples to lyse.
The FastPrep Family product line provides a powerful molecular tool for gaining insight into microbial communities in sediment samples. FastDNA and FastRNA kits for soil used in combination with the FastPrep-24™ or FastPrep-96™ instrument will help overcome any difficulties with complete lysis of all sediment bacteria and isolation of pure DNA and RNA.
Typical Soil Sample Recommendations
Sample Name Sample Type Quantity Lysing Matrix FastPrep Speed FastPrep Time
Sediment Sediment 500 mg E 5.5 2 x 40 sec
Sediment Marine Sediment 50 mg E 5.5 2 x 40 sec
Sediment Soil/Rock 50 mg E 5.5 2 x 30 sec
FastPrep Kits & Instruments
Purification Kits SKU Free Sample (Availability)
FastDNA and FastRNA kits for soil 116540600
FastRNA™ Pro Soil-Direct Kit
FastRNA™ Pro Soil-Indirect Kit
Publications and References
Thomsen, T.R., et al. (2001) Biogeochemical and Molecular Signatures of Anaerobic Methane Oxidation in a Marine Sediment. Applied and Environmental Microbiology 67, 1646-1656.
Tas, N., et al. (2011) Role of "Dehalococcoides" spp. In the Anaerobic Transformation of Hexachlorobenzene in European Rivers. Applied and Environmental Microbiology 77, 4437-4445.
Ye, W., et al. (2009) The vertical distribution of bacterial and archaeal communities in the water and sediment of Lake Taihu. FEMS Microbiology Ecology 70, 263-276.
Dang, H., et al. (2008) Diversity and spatialdistribution of sediment ammonia-oxidizing crenarchaeota in response to estuarine and environmental gradients in the Changjiang Estuary and East China Sea. Microbiology 154, 2084-2095.
Jorgensen, S.L., et al. (2012) Correlating microbial community profiles with geochemical data in highly stratified sediments from the Arctic Mid-Ocean Ridge. PNAS 109.
Swan, B.K., et al. (2010) Archaeal and Bacterial Communities Respond Differently to ENvironmental Gradients in Anoxic Sediments of a California Hypersaline Lake, the Salton Sea. Applied and Environmental Microbiology 76, 757-768.