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Urea,cell culture reagent, 100 g

Urea,cell culture reagent, 100 g

SKU: 02194762.1

Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide
CAS Number:
Molecular Formula:
Molecular Weight:
Beilstein Registry Number:
EC Number:
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Packaging size:
100 g
SKU 02194762.1
Alternate Names Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide
Application Notes Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Base Catalog Number 194762.1
Beilstein Registry Number 635724
Biochemical Physiological Actions Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Boiling Point Decomposes at 135 °C (275 °F)(Lit.)
CAS # 57-13-6
Density 1.335 g/mL at 25 °C (Lit.)
EC Number 200-315-5
Grade Cell Culture Grade
Melting Point 130 - 140 °C
Molecular Formula CH4N2O
Molecular Weight 60.06
Pack Size 100 g
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
pH 7.5 - 9.5 (7 M aq soln)
Purity ≥98%
RTECS Number YR6250000
Solubility Soluble in water (480 mg/mL, 8 M).
Typical Working Concentration The use of 2 g/L urea in the culture of <i>Kluyveromyces marxianus</i> to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance OD260 nm (7 M aq soln): ≤0.2; OD280 (7M aq soln): ≤0.2
Vapor Pressure 0.000016 hPa at 25 °C (Lit.)