Bes
Application Notes
The effect of BES and other zwitterionic buffers on the Bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.BES may be employed as binding buffer in modified Eagle's medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Buffer
| SKU | 02100927-CF |
| Alternate Names | N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid; N,N-Bis(2-hydroxyethyl)taurine |
| Application Notes | The effect of BES and other zwitterionic buffers on the Bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.BES may be employed as binding buffer in modified Eagle's medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers. |
| Base Catalog Number | 100927 |
| Beilstein Registry Number | 1781572 |
| CAS # | 10191-18-1 |
| EC Number | 233-465-5 |
| Format | Crystalline |
| Hazard Statements | H315-H319-H335 |
| Molecular Formula | C6H15NO5S |
| Molecular Weight | 213.248 g/mol |
| Personal Protective Equipment | Dust mask ,Eyeshields, Gloves |
| pKa | 7.1 at 25°C |
| Purity | ≥99% |
| RTECS Number | KI7363500 |
| Safety Symbol | GHS07 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [100927] lot ID: M4424](https://www.mpbio.com/static/version1744790984/frontend/Mpbio/default/en_US/images/document.png "MP COA [100927] lot ID: M4424")