IgG2B (Kappa) MOPC 141, mouse myeloma, purified, 1 mg

Product Description

Purified mouse myeloma protein IgG2B (Kappa) is purified mouse IgG₂b(j) and buffer. Mouse IgG2b is purified from clarified mouse MOPC 141 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography, and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | Positive control in antibody identification screening techniques

Specifications

SKU	0850331
Antibody Type	Monoclonal Antibody
Application Notes	This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Base Catalog Number	50331
Clone Name	MOPC 141
Concentration	Total Protein: 1.0 mg/mL; Volume: 1.0 mL
Conjugate	Unconjugated
Formulation Details	Purified
Host	Mouse
Isotype	IgG
Pack Size	1 mg
Preparation Method	Mouse IgG2b is purified from clarified mouse MOPC 141 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography, and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 μm filter and vialed. No preservative has been added.
Purity	Affinity Purified
Solubility	Soluble in Water
Specificity	The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2b. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG2b; no trace of IgG1; IgG2a; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.