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IgG2B (Kappa) MPOC 195, mouse myeloma, purified, 1 mg

IgG2B (Kappa) MPOC 195, mouse myeloma, purified, 1 mg
€230.00

Key features and details

Mouse IgG2b (j) is purified from clarified mouse MOPC 195 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02 M Tris, 0.15 M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

SKU: 0850330

Packaging size:
1 mg
Product Description

These myeloma proteins are purified from ascites by a combination of salt precipitation, ion exchange and bioaffinity chromatography. Purified proteins are dialyzed into 0.02M Tris, 0.14M sodium chloride, pH 8.1. Product is adjusted to 1.0 mg/ml, filtered, vialed, and stored frozen at -70°C. Product is shipped on dry ice. Mouse IgG2b (j) is purified from clarified mouse MOPC 195 ascites using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography, affinity chromatography and immunoabsorption. The product is dialyzed into 0.02M TRIS, 0.15M sodium chloride, pH 8.1, filtered through a 0.22 µm filter and vialed.

Application Notes

This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays

Specifications
SKU 0850330
Antibody Type Monoclonal Antibody
Application Notes This product is suitable for use as a positive control in antibody identification screening techniques and is recommended as an ELISA coating antigen and control.
Base Catalog Number 50330
Clone Name MOPC 195
Concentration Total Protein: 1.2 mg/mL
Conjugate Unconjugated
Host Mouse
Isotype IgG
Pack Size 1 mg
Solubility Soluble in Water
Specificity The total protein is determined by absorbance at 280nm. The product contains 1 mg of mouse IgG2b. The product is tested for purity at 1.0 mg/ml using immunoelectrophoresis. The product is mouse IgG2b; no trace of IgG1; IgG2a; IgG3; IgA; IgM or non-immunoglobulin serum proteins is detected.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificate of Analysis (COA)

Datasheet

Datasheet

Safety Data Sheets

English (United States)