SPINeasy 96-Well Plasmid Miniprep Kit enables a simple and high-throughput purification of plasmid DNA from bacterial hosts. This kit employs a silica-membrane spin plate in conjunction with an Alkaline Lysis procedure. It effectively captures plasmid DNA while swiftly eliminating undesired proteins and contaminants, completing the process in as little as 45 minutes. The highly purified plasmid DNA extracted with this kit is immediately ready for routine molecular biology laboratory applications such as endpoint PCR and restriction enzyme digestion.
Following are the results of purification of plasmid DNA from SPINeasy 96-Well Plasmid Miniprep Kit and competitor T (in 3 replicates) using either Centrifugation Protocol (figure 1) or Vacuum Protocol (figure 2). Plasmid DNA purified with SPINeasy 96-Well Plasmid Miniprep Kit were of high purity, and the yield were much higher than that of competitor T.
Figure 1. Agarose gel electrophoresis of plasmid DNA purified with SPINeasy 96-Well Plasmid Miniprep Kit (Lane 1-3) and Competitor T (Lane 4-6), using Centrifugation Protocol.
Figure 2. Agarose gel electrophoresis of plasmid DNA purified with SPINeasy 96-Well Plasmid Miniprep Kit (Lane 1-3) and Competitor T (Lane 4-6), using Vacuum Protocol.
Plasmid DNA purified using SPINeasy 96-Well Plasmid Miniprep Kit is also suitable for restriction enzyme digestion and endpoint PCR.
Figure 3. Plasmid DNA purified using SPINeasy 96-Well Plasmid Miniprep Kit can directly be used for restriction enzyme digestion and endpoint PCR. M: DNA marker, Lane 1: Plasmid DNA, Lane 2: Plasmid DNA linearized by restriction enzyme digestion, Lane 3: Negative control for endpoint PCR and Lane 4: Amplicon from endpoint PCR.
Application Notes
The SPINeasy® 96 Well Plasmid Miniprep Kit is for the purification of Plasmid DNA from bacterial hosts.
Usage Statement
Research Use Only (RUO)
Key Applications
Restriction enzyme digestion, end point PCR
| SKU | 116534-CF |
| Application Notes | The SPINeasy® 96 Well Plasmid Miniprep Kit is for the purification of Plasmid DNA from bacterial hosts. |
| Base Catalog Number | 6534096 |
| Product Line | SPINeasy |
| Components | Resuspension Buffer SN1 (30 mL), Alkaline Lysis Buffer SN2 (30 mL), Neutralization Buffer SN3 (42 mL), Wash Buffer SN1 (42 mL), Wash Buffer SN2 (9.6 mL), Elution Buffer SN (30 mL), RNase A (0.3 mL), Indicator Solution (0.15 mL), 96 DNA Plate G (1 ea) |
| Pack Size | 1 Box |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Sample Type | Yeast |
| Storage | Store at Room Temperature (15-25°C), Dry environment. |
| Usage Statement | Research Use Only (RUO) |
