DL-Dithiothreitol, ≥99.0%

Key features and details

Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE
Useful for stabilizing sulfhydryl containing enzymes
Less pungent and is less toxic than 2-mercaptoethanol

SKU: 02100597-CF

Synonyms

1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent

CAS Number:

578517

Molecular Formula:

C4H10O2S2

Beilstein Registry Number:

1719757

EC Number:

248-531-9

MDL Number:

MFCD00004877

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Product Description

Dl-Dithiothreitol

Application Notes

Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).DTT has been used:• as one of the reactants in the reduction and alkylation of as1-Casein, the major allergen of cow's milk.• as a component of medium for the demembranation and reactivation of spermatozoa.• to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.• as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Sulfhydryl reducing agent

Specifications

SKU	02100597-CF
Alternate Names	1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent
Application Notes	Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).DTT has been used:• as one of the reactants in the reduction and alkylation of as1-Casein, the major allergen of cow's milk.• as a component of medium for the demembranation and reactivation of spermatozoa.• to maintain stability of the enzyme as thiol effectively protects the active sites of the biocatalyst.• as a reducing agent to test the specificity of the reaction of N-Ethylmaleimide with sulfhydryl groups.
Base Catalog Number	100597
Beilstein Registry Number	1719757
CAS #	578517
EC Number	248-531-9
Format	Powder
Hazard Statements	H302-H315-H319-H335
Molecular Formula	C4H10O2S2
Personal Protective Equipment	Dust mask, Eyeshields, Faceshields, Gloves
Purity	≥99.0%
RTECS Number	XO8576500
Safety Symbol	GHS07
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.