Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse complement C3 and buffer salts.
Application Notes
Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting) and tissue staining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Immunofluorescence assays (IFA) | Flow cytometry
| SKU | 0855500 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting) and tissue staining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. |
| Base Catalog Number | 55500 |
| Concentration | Total Protein: 8.3 mg/mL; Antibody Protein: 4 mg/mL |
| Conjugate | FITC |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Goat |
| Pack Size | 2 mL |
| Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. |
| Purity | Purified |
| Solubility | Soluble in water |
| Species Reactivity | Mouse |
| Specificity | The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected. This product shows reactivity to mouse complement C3; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present. |
| Typical Working Concentration | Suggested working dilutions: 1:100 - 1:400 for Tissue Staining; 1:1,000 - 1:4,000 for ELISA |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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