Anti-HBc ELISA 4.0
The MP Diagnostics Anti-HBc ELISA 4.0 is a competitive immunoassay. The wells of the polystyrene microplate strips are coated with purified HBcAg specific to anti-HBc. Human serum or plasma, are incubated with the conjugate in these coated wells. The conjugate is a monoclonal anti-HBc conjugated to horseradish peroxidase. Anti-HBc, if present, will compete with the conjugate for HBcAg immobilised on the solid phase. If there is no anti-HBc present, the conjugate will bind with the immobilised HBcAg. After incubation, the wells are thoroughly washed to remove unbound materials. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well and hydrolysed by the bound conjugate. The presence of bound conjugate is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of sulphuric acid. The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is inversely proportional to the amount of antibodies present in the specimen.