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Enzyme Immunoassay (EIA/ELISA), Human

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HEV ELISA (IgG) (CE)

The MP Diagnostics HEV ELISA 4.0 is an enzyme-linked immunosorbent assay intended for the detection of total antibodies to Hepatitis E Virus in human serum or plasma. It is intended as a screening test, requiring repeat testing of initially reactive specimens.

HBc IgM ELISA 4.0

The MP Diagnostics (MPD) HBc IgM ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of IgM antibodies to Hepatitis B Virus Core Antigen (HBcAg) in human serum or plasma samples.

Anti-HBs ELISA 4.0

The MP Diagnostics (MPD) Anti-HBs ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of antibodies to Hepatitis B Virus Surface Antigen (anti-HBs) in human serum or plasma samples.

HIV-1/2 ELISA 4.0

The MP Diagnostics HIV-1/2 ELISA 4.0 is an antigen sandwich immunoassay. The wells of the polystyrene microplate strips are coated with recombinant HIV antigens (gp41, gp120, and gp-36) expressed in E.coli. The conjugate is based on a second set of recombinant HIV antigens expressing the same epitopes as the pre-coated antigens, which is conjugated to horseradish peroxidase. Human serum or plasma, are incubated in these coated wells. HIV-1/2 specific antibodies, if present, will bind to the antigens immobilised on the solid phase. After incubation, the wells are thoroughly washed to remove unbound materials and conjugate is added to the wells. The antigens of the conjugate will bind to any antigen-antibody complexes previously formed and excess unbound conjugate are removed by washing. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well. The presence of specific antibodies is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of sulphuric acid. The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is proportional to the amount of antibodies present in the specimen.

HEV IgM ELISA 3.0 (RUO)

The MPD HEV IgM ELISA 3.0 is an indirect immunoassay that utilizes a highly conserved conformational epitope (9) derived from open reading frame 2 (ORF 2) of the virus. The presence of IgM antibodies in the specimen is detected by monoclonal mouse anti-human IgM antibodies labelled with horseradish peroxidase.

HEV ELISA 4.0 (RUO)

A HEV ELISA that is highly sensitive and specific is needed for the detection of HEV total antibodies in human serum or plasma. The MPD HEV ELISA 4.0 utilises a proprietary recombinant antigen, which is highly conserved between different HEV strains (22,23,24), to detect the presence of specific antibodies including IgG, IgM and IgA against HEV.

Anti-HBs ELISA 4.0

The MP Diagnostics Anti-HBs ELISA 4.0 is a double antibody sandwich immunoassay. The wells of the polystyrene microplate strips are coated with recombinant HBsAg specific to anti-HBs. Human serum or plasma, are incubated in these coated wells together with a second HBsAg labelled with horseradish peroxidase. Anti-HBs, if present, forms a specific immunocomplex and is captured on the solid phase. After incubation, the wells are thoroughly washed to remove unbound materials. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well. The presence of specific antibodies is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of sulphuric acid. The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is proportional to the amount of antigens present in the specimen.

Anti-HBc ELISA 4.0

The MP Diagnostics Anti-HBc ELISA 4.0 is a competitive immunoassay. The wells of the polystyrene microplate strips are coated with purified HBcAg specific to anti-HBc. Human serum or plasma, are incubated with the conjugate in these coated wells. The conjugate is a monoclonal anti-HBc conjugated to horseradish peroxidase. Anti-HBc, if present, will compete with the conjugate for HBcAg immobilised on the solid phase. If there is no anti-HBc present, the conjugate will bind with the immobilised HBcAg. After incubation, the wells are thoroughly washed to remove unbound materials. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well and hydrolysed by the bound conjugate. The presence of bound conjugate is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of sulphuric acid. The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is inversely proportional to the amount of antibodies present in the specimen.

Yersinia enterocolitica IgM ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Yersinia enterocolitica IgA ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Yersinia enterocolitica IgG ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Varicella zoster IgM ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Varicella zoster IgA ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Varicella zoster IgG ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Tuberculosis (Mycobacterium tuberculosis) IgM ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Tuberculosis (Mycobacterium tuberculosis) IgA ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Tuberculosis (Mycobacterium tuberculosis) IgG ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.

Toxoplasma gondii IgM ELISA Kit (RUO)

The kit is ready to use and includes the following components: 96-microwell antibody coated plate, Enzyme Conjugate, Sample Diluent, two Controls, 4 Calibrators, Wash Buffer, TMB Reagent, and Stop Solution. Kit has a lead time of 1 week from order receipt.

Toxoplasma gondii IgG ELISA Kit (RUO)

The kit is ready to use and includes the following components: 96-microwell antibody coated plate, Enzyme Conjugate, Sample Diluent, two Controls, 4 Calibrators, Wash Buffer, TMB Reagent, and Stop Solution. Kit has a lead time of 1 week from order receipt.

Tetanus (Chostridium tetani) IgG ELISA Kit (CE)

The kit is ready to use and includes the following components: Microtiter Strips, 4 calibrators, Enzyme Conjugate, Substrate, Stop Solution, Sample Diluent, Washing Buffer, Plastic Foils, and a Plastic Bag. Kit has a lead time of 3-4 weeks from order receipt.