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Enzyme Immunoassay (EIA/ELISA), EIA/ELISA, Hepatitis B Virus (HBV)

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HBsAg ELISA 4.0

The MP Diagnostics (MPD) HBsAg ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of Hepatitis B Virus Surface Antigen (HBsAg) in human serum or plasma samples.

HBsAg ELISA 4.1 (CE)

The MP Diagnostics HBsAg ELISA 4.1 is an enzymelinked immunosorbent assay (ELISA) intended for qualitative detection of HBsAg in human serum or plasma. It is intended for screening of blood donors and for diagnosing of patients related to infection with hepatitis B virus.

Anti-HBs ELISA 4.0 (RUO)

The MP Diagnostics Anti-HBs ELISA 4.0 is an enzyme linked immunosorbent assay (ELISA) for in vitro qualitative detection of antibodies to hepatitis B virus surface antigen (anti-HBs) in human serum or plasma. This kit is supplied for research purposes only. It is not intended for use in the diagnosis or prognosis of disease. In particular this test cannot be used to evaluate blood specimens for the purpose of donor screening or confirmatory diagnostic.

Anti-HBs ELISA 4.0

The MP Diagnostics (MPD) Anti-HBs ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of antibodies to Hepatitis B Virus Surface Antigen (anti-HBs) in human serum or plasma samples.

Anti-HBe ELISA 4.0 (RUO)

The MP Diagnostics HBeAg ELISA 4.0 is an enzymelinked immunosorbent assay (ELISA) for qualitative detection of hepatitis B virus e antigen (HBeAg) in human serum or plasma. This kit is supplied for research purposes only. It is not intended for use in the diagnosis or prognosis of disease. In particular this test cannot be used to evaluate blood specimens for the purpose of donor screening or confirmatory diagnostic.

Anti-HBe ELISA 4.0

The MP Diagnostics (MPD) Anti-HBe ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of antibodies to Hepatitis B Virus e Antigen (HBeAg) in human serum or plasma samples.

HBeAg ELISA 4.0

The MP Diagnostics (MPD) HBeAg ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of Hepatitis B Virus e Antigen (HBeAg) in human serum or plasma samples.

HBsAg ELISA 4.0 (RUO)

The MPD HBsAg ELISA 4.0 is a double antibody sandwich immunoassay that utilises monoclonal antibodies speci fic to HBsAg. The presence of HBsAg in a specimen is detected by a secondary antibody conjugated to horseradish peroxidase. It is intended as a qualitative enzyme-linked immunosorbent assay for the detection of HBsAg found in human serum or plasma. It is intended as a first line screening, requiring repeat testing of initially reactive specimens and confi rmation of repeat reactive specimens by supplementary assays.

HBeAg ELISA 4.0 (RUO)

The MPD HBeAg ELISA 4.0 is a double antibody sandwich immunoassay that utilises monoclonal antibodies specific to HBeAg. The presence of HBeAg in a specimen is detected by a secondary antibody conjugated to horseradish peroxidase. It is intended as a qualitative enzyme-linked immunosorbent assay for the detection of HBeAg found in human serum or plasma. It is intended as a first line screening, requiring repeat testing of initially reactive specimens and confirmation of repeat reactive specimens by supplementary assays.

HBc IgM ELISA 4.0

The MP Diagnostics (MPD) HBc IgM ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of IgM antibodies to Hepatitis B Virus Core Antigen (HBcAg) in human serum or plasma samples.

Anti-HBs ELISA 4.0

The MP Diagnostics (MPD) Anti-HBs ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of antibodies to Hepatitis B Virus Surface Antigen (anti-HBs) in human serum or plasma samples.

Anti-HBs ELISA 4.0

The MP Diagnostics Anti-HBs ELISA 4.0 is a double antibody sandwich immunoassay. The wells of the polystyrene microplate strips are coated with recombinant HBsAg specific to anti-HBs. Human serum or plasma, are incubated in these coated wells together with a second HBsAg labelled with horseradish peroxidase. Anti-HBs, if present, forms a specific immunocomplex and is captured on the solid phase. After incubation, the wells are thoroughly washed to remove unbound materials. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well. The presence of specific antibodies is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of sulphuric acid. The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is proportional to the amount of antigens present in the specimen.

Anti-HBc ELISA 4.0

The MP Diagnostics Anti-HBc ELISA 4.0 is a competitive immunoassay. The wells of the polystyrene microplate strips are coated with purified HBcAg specific to anti-HBc. Human serum or plasma, are incubated with the conjugate in these coated wells. The conjugate is a monoclonal anti-HBc conjugated to horseradish peroxidase. Anti-HBc, if present, will compete with the conjugate for HBcAg immobilised on the solid phase. If there is no anti-HBc present, the conjugate will bind with the immobilised HBcAg. After incubation, the wells are thoroughly washed to remove unbound materials. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well and hydrolysed by the bound conjugate. The presence of bound conjugate is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of sulphuric acid. The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is inversely proportional to the amount of antibodies present in the specimen.

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