IgG, rabbit, purified, IgG, 50 mg

IgG, rabbit, purified, IgG, 50 mg

Synonyms IgG from rabbit serum; Rabbit IgG

MDL Number: MFCD00165673

Packaging size: 50 mg

SKU 0855944
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Description

Product Description

Product is the lyophilized powder of rabbit IgG and buffer salts. Rabbit IgG is purified from pooled rabbit serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative added.

Application Notes

Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rabbit IgG was used to reduce non-specific binding in immunoprecipitation of protein extracts and as control antibody in radioimmunoassay, immunocytochemistry and immunohistochemistry. Rabbit IgG was also used as a standard to demonstrate the performance of land-contrast (LC) BioCD (protein array) and in neutralization assays. It is suitable for use as a positive control in antibody identification screening techniques.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | ELISA | Dot immunobinding | Immunoblot | Immunodiffusion | Immunoelectrophoresis

Specifications
SKU 0855944
Alternate Names IgG from rabbit serum; Rabbit IgG
Antibody Type Polyclonal Antibody
Application Notes Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rabbit IgG was used to reduce non-specific binding in immunoprecipitation of protein extracts and as control antibody in radioimmunoassay, immunocytochemistry and immunohistochemistry. Rabbit IgG was also used as a standard to demonstrate the performance of land-contrast (LC) BioCD (protein array) and in neutralization assays. It is suitable for use as a positive control in antibody identification screening techniques.
Base Catalog Number 55944
Concentration Total Protein: 18.2 mg/mL; Volume: 3 mL
Conjugate Unconjugated
Formulation Details Lyophilized
Host Rabbit
Isotype IgG
Pack Size 50 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Preparation Method Rabbit IgG is purified from pooled rabbit serum using multi-step procedures which may include salt fractionation, gel filtration, ion-exchange chromatography and immunoabsorption. The product is dialyzed into 0.01M sodium phosphate, 0.07M sodium chloride, pH 7.3, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative added.
Purity > 99%; lyophilized
Solubility Soluble in water
Source Rabbit
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.