Protease inhibitor cocktail kit, 1 kit

Protease inhibitor cocktail kit, 1 kit

CAS Number: Not applicable

EC Number: 200-664-3 MDL Number: MFCD00677817

Packaging size: 1 kit

SKU 0215883701
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Description

Product Description

Protease Inhibitor Cocktail Kit

Application Notes

Protease Inhibitor Cocktail Kit contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. It has been tested on a wide variety of cells and tissue extracts to isolate/maintain intact proteins without degradation.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Protease Inhibitor

Specifications
SKU 0215883701
Application Notes Protease Inhibitor Cocktail Kit contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. It has been tested on a wide variety of cells and tissue extracts to isolate/maintain intact proteins without degradation.
Base Catalog Number 15883701
Biochemical Physiological Actions Proteases are enzymes which direct their action upon proteins, cleaving their targets into smaller fragments consisting of polypeptides, peptides and amino acids. There are a myriad of proteases active against practically all proteins. Some proteases are quite specific, while others cleave many different proteins. MP Protease Inhibitor Cocktail covers a broad spectrum of protese inhibitors.
CAS # Not applicable
Components 193503-AEBSF(40 mg), 151553- Leupeptin (0.1 mg), 195368- Pepstatin(0.1 mg),195173- EDTA-Na2(500 mg)
EC Number 200-664-3
Hazard Statements Class 8
Pack Size 1 kit
Preparation Method Place the contents of all four vials in one volumetric flask or other suitable container. Add water or buffer of choice to 100 mL. More concentrated solutions can be prepared as desired; however, leupeptin and pepstatin A will create more of a suspension at concentrations other than the suggested amount. The prepared solution is stable for 60-90 days when stored at 5 °C. Do not freeze the prepared solution, and avoid repeated freeze-thaw cycles.
Typical Working Concentration Typical working concentration for 100 mL of inhibitor cocktail: AEBSF Hydrochloride:50-400 mg/mL; EDTA disodium dihydrate:0.2-25 mg/mL; Leupeptin Hemisulfate: 0.5-1 mg/mL; Pepstatin A: 0.5-1 mg/mL
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.