PhoenIX Midiprep Sample Kit, 25 Preps
PhoenIX™ Midiprep Kit provides gravity-flow, anion-exchange-based plasmid DNA purification with bacterial lysate clearing by filtration. The kits deliver DNA yields of up to 100 μg of ultrapure plasmid DNA from 15-25 mL of E. coli cells culture. Cleared bacterial lysate is poured through the filter and onto the PhoenIX™ Maxi Column in a single step for rapid purification by gravity flow. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation. Plasmids obtained are suitable for transfection, microinjection, sequencing, amplification, digestion, etc. Protocols for high copy and low copy plasmids are included in the instruction manual.
PhoenIX™ Midiprep Kit is designed for fast purification of up to 100 ug transfection-grade plasmid or cosmid DNA.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Purification of plasmid
|Application Notes||PhoenIX™ Midiprep Kit is designed for fast purification of up to 100 ug transfection-grade plasmid or cosmid DNA.|
|Base Catalog Number||2075200|
|Components||RNase A, Cell Resuspension Buffer, Lysis Buffer, Neutralization Buffer, Equilibration Solution, Column Wash Buffer, Elution Buffer, TE Buffer, PhoenIXTM Midi Columns.|
|Pack Size||25 Preps|
|Personal Protective Equipment||Eyeshields, Gloves|