PhoenIX™ Filter Maxiprep Kit provides anion-exchange-based plasmid DNA purification with bacterial lysate clearing by filtration. The kits deliver DNA yields of up to 500 μg of ultrapure plasmid DNA from 100 - 500 mL of bacterial culture. Cleared bacterial lysate is poured through the filter and onto the PhoenIXTM Maxi Column in a single step for rapid purification by gravity flow. The purified DNA is equivalent to that obtained by 2 x CsCl gradient centrifugation. Plasmids obtained are suitable for transfection, microinjection, sequencing, amplification, digestion, etc. Protocols for high copy and low copy plasmids are included in the instruction manual.
PhoenIX™ Maxiprep Kit is designed for fast purification of up to 500 ug transfection-grade plasmid or cosmid DNA.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Purification of plasmid
|Application Notes||PhoenIX™ Maxiprep Kit is designed for fast purification of up to 500 ug transfection-grade plasmid or cosmid DNA.|
|Base Catalog Number||2075300|
|Components||RNase A, Cell Resuspension Buffer, Lysis Buffer, Neutralization Buffer, Equilibration Solution, Column Wash Buffer, Elution Buffer, TE Buffer, PhoenIXTM Maxi Columns|
|Pack Size||25 Preps|
|Personal Protective Equipment||Eyeshields, Gloves|