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氮蓝四唑, 分子生物学试剂

氮蓝四唑, 分子生物学试剂

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CAS:298-83-9 氯化硝基四氮唑蓝, 氮蓝四唑, 硝基蓝四氮唑, 硝基BT, 氯化硝基四氮唑兰, 硝基氯化四氮唑蓝, 氮四唑蓝, 氯化四唑氮蓝, NBT 氮蓝四唑

SKU: 02193999-CF

Synonyms
2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
CAS Number:
298-83-9
Molecular Formula:
C40H30N10O6 • 2Cl
Molecular Weight:
817.644 g/mol
Beilstein Registry Number:
4115923
EC Number:
206-067-4
MDL Number:
MFCD00012159
Product Description

P-Nitro Blue Tetrazolium Chloride

Application Notes

p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Colorimetric detection of alkaline phosphatase conjugates

Specifications
SKU 02193999-CF
Alternate Names 2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride; 3,3'-(3,3'-Dimethoxy-4,4'-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]; p-Nitro-Blue tetrazolium chloride; p-Nitrotetrazolium blue; NBT; Nitro BT
Application Notes p-nitro blue tetrazolium is used for the detection of alkaline phosphatase on western blots. It has also been used as a redox indicator for other enzymatic reactions including dehydrogenases, threonine deaminase, glucose-6-phosphate dehydrogenase, phosphofructokinase on polyacrylamide gels, oxidases on polyacrylamide gels, and pentose shunt dehydrogenases. Redox and halfwave potentials have been determined for p - nitro blue tetrazolium. It has also been used as a colorimetric indicator of bacterial infection in blood samples.
Base Catalog Number 193999
Beilstein Registry Number 4115923
CAS # 298-83-9
EC Number 206-067-4
Extinction Coefficient 260 nm: E1% = 740 (in water) (Lit.)
Format Powder
Grade Molecular Biology Grade
Hazard Statements H302
Molecular Formula C40H30N10O6 • 2Cl
Molecular Weight 817.644 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
RTECS Number XF8045000
Safety Symbol GHS07
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance 255 ± 3 nm ( λ max (methanol))
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