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β-烟酰胺腺嘌呤二核苷酸, 氧化型, 细胞培养级, ≥98%

β-烟酰胺腺嘌呤二核苷酸, 氧化型, 细胞培养级, ≥98%

CN¥1,008.15

Key features and details

CAS:53-84-9 烟酰胺腺嘌呤双核苷酸, β-辅酶A β-烟酰胺腺嘌呤二核苷酸, 氧化型

SKU: 02194714-CF

Synonyms
β-DPN; NAD; Nadide; β-NAD; Diphosphopyridine nucleotide; DPN; Coenzyme 1; Cozymase; Nadide; Adenosine 5'-(trihydrogen diphosphate) P'->5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium inner salt; 3-carbamoyl-1-β-D-ribofuranosylpyridinium hydroxide 5'->5'- ester with adenosine 5'-(trihydrogen pyrophosphate) inner salt
CAS Number:
53-84-9
Molecular Formula:
C21H27N7O14P2·×H2O
Molecular Weight:
663.43 g/mol
EC Number:
200-184-4
MDL Number:
MFCD00036253
Product Description

β-Nicotinamide Adenine Dinucleotide, Oxidized Form

Application Notes

Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Fluorescent measurement of the oxidized or reduced pyridine nucleotides

Specifications
SKU 02194714-CF
Alternate Names β-DPN; NAD; Nadide; β-NAD; Diphosphopyridine nucleotide; DPN; Coenzyme 1; Cozymase; Nadide; Adenosine 5'-(trihydrogen diphosphate) P'->5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium inner salt; 3-carbamoyl-1-β-D-ribofuranosylpyridinium hydroxide 5'->5'- ester with adenosine 5'-(trihydrogen pyrophosphate) inner salt
Application Notes Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Base Catalog Number 194714
Biochemical Physiological Actions Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.
CAS # 53-84-9
EC Number 200-184-4
Extinction Coefficient EmM = 18.0 (260 nm, pH 7)(Lit.)
Format Powder
Grade Cell Culture Grade
Molecular Formula C21H27N7O14P2·×H2O
Molecular Weight 663.43 g/mol
Purity ≥98%
RTECS Number UU3450000
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance λ max (water) 259 ± 3 nm
Citations
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