Mouse IgG, lyophilized

Mouse IgG, lyophilized

Synonyms Mouse IgG; IgG from mouse serum

MDL Number: MFCD00212351

SKU 0864146-CF
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Description

Product Description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Mouse IgG is purified from normal mouse serum.

Application Notes

Mouse IgG is useful for structural and biological studies, for use as reference standards or as starting material for the preparation of immunogen. Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays

Specifications
SKU 0864146-CF
Alternate Names Mouse IgG; IgG from mouse serum
Antibody Type Polyclonal Antibody
Application Notes Mouse IgG is useful for structural and biological studies, for use as reference standards or as starting material for the preparation of immunogen. Purified rabbit IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Mouse IgG was used as standard in ELISA and chromatin immunoprecipitation assay. It was used to coat nano particles in the development of biosensors.
Base Catalog Number 641461,641462
Conjugate Unconjugated
Formulation Details Lyophilized | Each vial contains 10 mg of lyophilized protein based on; E<sup>1%</sup><sup>280 nm</sup> = 14. This product was lyophilized from 0.01M sodium phosphate; 0.07M sodium chloride; pH 7.3. There is no preservative added. | It is supplied as purified immunogen in either Tris or phosphate buffered saline.
Host Mouse
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Preparation Method This immunoglobulin was prepared from mouse serum using current chromatographic techniques. A quantitative protein analysis as well as immunoelectrophoresis has been applied to determine identity of the product.
Purity > 95% by SDS-PAGE
Solubility Soluble in water
Specificity By immunoelectrophoresis: Antibody vs. rabbit anti-mouse serum - shows one band. The product is mouse IgG with no trace of IgA; IgM; or non-immunoglobulin serum proteins detected.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.