Cart
 
Inquire

FastDNA 组织和昆虫DNA提取试剂盒(96深孔板), 2 x 96份装

FastDNA 组织和昆虫DNA提取试剂盒(96深孔板), 2 x 96份装

CN¥14,746.50

Key features and details

用于从动物和昆虫样品中快速、高通量地分离出适合PCR的基因组DNA。

SKU: 119696500

Synonyms
96 well DNA purification kit
Packaging size:
2 x 96 Preps
Product Description

Rapid, High-Throughput Isolation of PCR-Ready Genomic DNA from Animal and Insect Samples The FastDNA™-96 Animal and Insect DNA Kit is designed for the simple and rapid isolation of PCR-quality DNA from genomic, viral and mitochondrial DNA from fresh or frozen tissue samples, insects, and arthropods in approximately 40 minutes. Animal tissues (brain, heart, kidney, liver, bone, etc), cultured mammalian cells, whole blood, as well as D. melanogaster, mosquitoes, bees, lice, and ticks are easily lysed in approximately 60 seconds with the FastPrep-96™ Instrument. Up to 10 mg of fresh, frozen, or stored mammalian tissue or insects are placed into each 1.2 ml tube of a FastDNA-96™ Lysing Matrix Rack ( 96-deep well plate) containing 2.0 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse all cell and tissue types whether mammalian or invertebrate, while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, and spin column plates are used to simplify liquid handling. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis, arrays, genotyping and PCR (A260/A280 ratios ≥ 1.8). Yield is typically 1-5 μg of DNA per mg tissue (3-7μg ofDNA per 100 μl whole blood) eluted in 50-100 μl of elution solution (25 mL minimum.) This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.

Application Notes

The FastDNA-96® Tissue & Insect DNA Kit is designed for the simple and rapid isolation of PCR-quality DNA from genomic, viral and mitochondrial DNA from fresh or frozen tissue samples, insects, and arthropods in approximately 40 minutes. The DNA then isolated and purified using spin column plate technologies is now ideal for downstream molecular-based applications including PCR, array, genotyping etc.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

High-Throughput, Rapid Isolation of PCR-Ready Genomic DNA from Animal and Insect Samples using the FastPrep-96™ System

Specifications
SKU 119696500
Alternate Names 96 well DNA purification kit
Application Notes The FastDNA-96® Tissue & Insect DNA Kit is designed for the simple and rapid isolation of PCR-quality DNA from genomic, viral and mitochondrial DNA from fresh or frozen tissue samples, insects, and arthropods in approximately 40 minutes. The DNA then isolated and purified using spin column plate technologies is now ideal for downstream molecular-based applications including PCR, array, genotyping etc.
Base Catalog Number 9696500
Brand FastDNA™
Components FastDNA-96&trade||Lysing Matrix Rack (2.0 mm Beads) 2 x 96-well rack||Lysis Buffer 2 x 40 ml||Tissue/Insect DNA Binding Solution 150 ml||Binding Plate Pre-Wash Buffer 50 ml||Tissue/Insect DNA Wash Buffer 100 ml||Elution Solution 2 x 10 ml||Deep-Well Plate 2 each
FastPrep Instruments Models 2 x 96 Well Adapter
Hardness Mohs 9, Rockwell A >87, Vickers >1350
Pack Size 2 x 96 Preps
Personal Protective Equipment Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Specific Gravity 6.02
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)

Brochures

Brochures
English (United States)
Brochures
English (United States)