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抑肽酶

抑肽酶

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Key features and details

CAS:9087-70-1 组抑肽酶;抑肽酶(来源于牛肺,胰蛋白酶抑制剂);APROTININ 蛋白酶抑制剂(抑肽酶);抑酶肽;重组胰蛋白酶抑制剂;抑肽酶;抗蛋白酶肽(牛肺);抑肽酶(牛肺) 抑肽酶

SKU: 02190382-CF

Synonyms
Pancreatic Basic Trypsin Inhibitor polypeptide; Antagosan; Antikrein; Trasylol; Zymofren; Antagosan; Anikrein; Antilysin(e); BPTI; Bayer A 128; Kallikrein-trypsin inactivator; Fosten; Iniprol; Kunitz protease inhibitor; Onquinin; Repulson; RP-9921; Ryker 52G; Triazinin; Zymofren
CAS Number:
9087-70-1
Molecular Formula:
C284H432N84O79S7
Molecular Weight:
6511.509 g/mol
EC Number:
232-994-9
MDL Number:
MFCD00130541
Product Description

Aprotinin

Application Notes

Small amounts of aprotinin can be added to tubes of drawn blood to enable laboratory measurement of rapidly degraded proteins such as glucagon. Aprotinin can be labelled with fluorescein isothiocyanate. The conjugate retains its antiproteolytic and carbohydrate-binding properties and has been used as a fluorescent histochemical reagent for staining glycoconjugates (mucosubstances) that are rich in uronic or sialic acids. In cell biology aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues .

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Enzyme inhibitor

Specifications
SKU 02190382-CF
Alternate Names Pancreatic Basic Trypsin Inhibitor polypeptide; Antagosan; Antikrein; Trasylol; Zymofren; Antagosan; Anikrein; Antilysin(e); BPTI; Bayer A 128; Kallikrein-trypsin inactivator; Fosten; Iniprol; Kunitz protease inhibitor; Onquinin; Repulson; RP-9921; Ryker 52G; Triazinin; Zymofren
Application Notes Small amounts of aprotinin can be added to tubes of drawn blood to enable laboratory measurement of rapidly degraded proteins such as glucagon. Aprotinin can be labelled with fluorescein isothiocyanate. The conjugate retains its antiproteolytic and carbohydrate-binding properties and has been used as a fluorescent histochemical reagent for staining glycoconjugates (mucosubstances) that are rich in uronic or sialic acids. In cell biology aprotinin is used as an enzyme inhibitor to prevent protein degradation during lysis or homogenization of cells and tissues .
Base Catalog Number 190382
Biochemical Physiological Actions Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.
CAS # 9087-70-1
EC Number 232-994-9
Extinction Coefficient 8.3 (water)(280 nm)
Grade Analytical Grade
Isoelectric Point 10.5 10 (Lit.)
Melting Point >100 °C
Molecular Formula C284H432N84O79S7
Molecular Weight 6511.509 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
RTECS Number YN5080000
Source Bovine Lung
Specific Activity ~6000 KIU/mg
Typical Working Concentration Equimolar with protease (1-2 ug/mL).
Unit Definition KIU: One KIU (Kallikrein Inactivating Unit) is identical to the quantity of protease inhibitor that has the ability to inhibit 2 Kallikrein units by 50% under optimal conditions.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance 277 ± 5 nm λmax (water)
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