Cart
 
In Stock

酵母裂解酶 100T(100,000 U/g)

酵母裂解酶 100T(100,000 U/g)

CN¥13,399.15

Key features and details

  • Used to lyse living yeast cell walls to produce protoplast or spheroplast
  • Can be used for glucan hydrolysis.

SKU: 083209-CF

Product Description

Zymolyase-100T is an enzyme preparation from a submerged culture of Arthrobacter luteus which effectively lyses cell walls of viable yeast cells. This preparation is lyophilized enzyme partially purified by affinity chromatography and contains 100,000 units enzyme/g. The primary enzyme in this preparation responsible for lysis of viable yeast cells in ß-1,3-glucan laminaripentaohydrolase. It hydrolyzes glucose polymers at the ß-1,3-glucan linkages releasing laminaripentaose as the principal product.

Application Notes

Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase-1T has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than .5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer solution (pH 7.5) which contains 5% glucose. To remove insoluble substances, filter through a sterile filter. Do not use Nitrocellulose filters. Dilute sterile enzyme suspension on to desired concentration.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Yeast Cell Culture | Used to lyse cell wall of yeast

Specifications
SKU 083209-CF
Application Notes Zymolyase 100T is used to lyse living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. It may also be used for glucan hydrolysis. Zymolase-1T has a low solubility. Use as a suspension. If it is necessary to make a sterile enzyme solution of more than .5%, make a 2% enzyme stock solution by dissolving Zymolase -1T in a buffer solution (pH 7.5) which contains 5% glucose. To remove insoluble substances, filter through a sterile filter. Do not use Nitrocellulose filters. Dilute sterile enzyme suspension on to desired concentration.
Base Catalog Number 320931,320932
Formulation Details Lyophilized
pH Optimum pH and temperature: pH 7.5, 35 °C for lysis of viable yeast cells; pH 6.5, 45 °C for hydrolysis of yeast glucan; pH Range: Stable through pH ranges 5-10
Protein or Enzyme Type Yeast Lytic Enzymes
Source Arthrobacter luteus
Specific Activity minimum of 100,000 units/g
Specificity The lytic spectrum of this enzyme includes organisms from the following genera: Ashbya; Candida; Debaryomyces; Eremothecium; Endomyces; Hansenula; Hanseniaspora; Kloekera; Kluyveromyces; Lipomyces; Metschikowia; Pichia; Pullularia; Torulopsis; Saccharomyces; Saccharomcopsis; Saccharomycodes; Schwannimomyces and others.
Unit Definition The amount of activity causing a decrease in absorbance at 800 nm of 30%.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Citations
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)

Material Safety Data Sheet

Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
English (United Kingdom)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)

Brochures

Brochures
English (United States)
Brochures
English (United States)
Brochures
English (United States)