Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.
Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Purification of DNA | Analysis of RNA sequences | RNase protection assays | Hydrolyze RNA contained in protein samples
|Alternate Names||Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase|
|Application Notes||Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.|
|Base Catalog Number||193980|
|Biochemical Physiological Actions||Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.|
|Extinction Coefficient||E1%280 = ~7.3|
|Format||Lyophilized powder, Salt-free and protease-free|
|Grade||Molecular Biology Grade|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|pH||Optimum pH: 7.0 - 7.5|
|Preparation Method||Chromatographically Purified|
|Solubility||Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.|
|Specific Activity||≥70 Kunitz units/mg|
|Specificity||The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.|
|Unit Definition||One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C at pH 5.0.|
|Usage Statement||Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.|