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核糖核酸酶 A

核糖核酸酶 A

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Key features and details

CAS:9001-99-4 核糖核酸酶属于胰腺核糖核酸酶家族。 RNase A是一种内切核糖核酸酶,攻击嘧啶核苷酸的3¢磷酸,将pG-pG-pC-pA-pG的序列切割,得到pG-pG-pCp和A-pG。

SKU: 02193980-CF

Synonyms
Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
CAS Number:
9001-99-4
EC Number:
232-646-6
MDL Number:
MFCD00132181
Product Description

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.

Application Notes

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Purification of DNA | Analysis of RNA sequences | RNase protection assays | Hydrolyze RNA contained in protein samples

Specifications
SKU 02193980-CF
Alternate Names Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase
Application Notes Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Base Catalog Number 193980
Biochemical Physiological Actions Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.
CAS # 9001-99-4
EC Number 232-646-6
Extinction Coefficient E1%280 = ~7.3
Format Lyophilized powder, Salt-free and protease-free
Grade Molecular Biology Grade
Hazard Statements H317
Isoelectric Point 9.45
Personal Protective Equipment Eyeshields, Gloves, respirator filter
pH Optimum pH: 7.0 - 7.5
Preparation Method Chromatographically Purified
RTECS Number RF0760000
Solubility Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Source Bovine Pancrease
Specific Activity ≥70 Kunitz units/mg
Specificity The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
Unit Definition One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C at pH 5.0.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Documents

Certificates of Analysis

Certificates of Analysis
English (United States)
Certificates of Analysis
English (United States)
Certificates of Analysis
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Certificates of Analysis
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Certificates of Analysis
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Material Safety Data Sheet

Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)
Material Safety Data Sheet
English (United States)