An aminonucleoside antibiotic that is toxic to both prokaryotic and eukaryotic cells. It causes premature chain termination in protein synthesis and is widely used in cell culture as a selection agent.
Puromycin can be used in vitro as a selection agent for cells transfected with puromycin N-acetyl transferase gene (pac). It causes premature chain termination in protein synthesis, and is an inhibitor of aminopeptidase and enkephalinase.
Inhibitor of aminopeptidase and enkephalinase.
|Alternate Names||Stylomycin ; 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine; 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride|
|Application Notes||Puromycin can be used in vitro as a selection agent for cells transfected with puromycin N-acetyl transferase gene (pac). It causes premature chain termination in protein synthesis, and is an inhibitor of aminopeptidase and enkephalinase.|
|Base Catalog Number||194539|
|Beilstein Registry Number||3853613|
|Biochemical Physiological Actions||Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3'-terminal end of the aminoacyl-tRNA.Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.|
|Grade||Cell Culture Grade|
|Personal Protective Equipment||Dust mask , Eyeshields, Gloves|
|Solubility||Soluble (NTP, 1992)|
|Typical Working Concentration||recommended working concentration in eukaryotic cell culture is 1-10 µg/mL|
|UV Visible Absorbance||λ max (0.1N HCl) 268 ± 2 nm|