Anti-human fiberonectin, goat antiserum, 60.2mg/ml, 2 mL

Anti-human fiberonectin, goat antiserum, 60.2mg/ml, 2 mL

Packaging size: 2 ml

SKU 0855125
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Description

Product Description

Product is the lyophilized powder of goat antiserum to human fibronectin and buffer salts.

Application Notes

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is recommended for detection of Fibronectin of mouse, rat and human origin by Western Blotting, immunoprecipitation [1-2 ug per 100-500 ug of total protein (1ml ofcell lysate)], immunofluorescence, immunohistochemistry(including paraffin-embedded sections) and solid phase ELISA.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Precipitating antibody | Immunofluorescence | Immunohistochemistry

Specifications
SKU 0855125
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is recommended for detection of Fibronectin of mouse, rat and human origin by Western Blotting, immunoprecipitation [1-2 ug per 100-500 ug of total protein (1ml ofcell lysate)], immunofluorescence, immunohistochemistry(including paraffin-embedded sections) and solid phase ELISA.
Base Catalog Number 55125
Concentration Total Protein: 60.2 mg/mL
Host Goat
Pack Size 2 ml
Preparation Method The antiserum is prepared by hyperimmunizing goats with highly purified human fibronectin; antiserum is delipidated and made specific by solid-phase absorption. The resulting antiserum is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 um filter, vialed and lyophilized.
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Sterilization of Solutions Filtered through a 0.22 um filter
Typical Working Concentration Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 1:2,000 for ELISA
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.