Glucose-6-phosphate dehydrogenase (G-6-PDH) was used as a model to test the effect of seed protein fractions on enzyme protection during dehydration. G-6-PDH has been utilized in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.
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Enzyme in pentose phosphate pathway
|Alternate Names||G-6-Pdase; G-6-P-DH|
|Application Notes||Glucose-6-phosphate dehydrogenase (G-6-PDH) was used as a model to test the effect of seed protein fractions on enzyme protection during dehydration. G-6-PDH has been utilized in assays for nicotinamide adenine dinucleotide and tissue pyridine nucleotides.|
|Base Catalog Number||151187|
|Biochemical Physiological Actions||Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.|
|Extinction Coefficient||E0.1% = 1.15 (280.5 nm, 0.09 M Tris-HCl, pH 7.2)(Lit.)|
|Isoelectric Point||4.6 (Lit.)|
|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge|
|Specific Activity||≥400 u/mg solid|
|Specificity||Either NAD+ or NADP+ serves as coenzyme; the reaction velocity with NAD+ being approximately 1.8 times greater than with NADP+ p-Glucose-6-phosphate is a preferential substrate for the enzyme; although p-Glucose reacts slowly; Fructose-6-phosphate; fructose-1; 6-diphoshate and ribose-5-phosphate are not considered to be substrates.|
|Unit Definition||One unit will reduce 1.0 µmole of NAD+ per minute at pH 7.8 and 25 °C.|