E-64

E-64

Synonyms [L-3-trans-Carboxyoxiran-2-carbonyl]-L-leucyl-agmatin; trans-Epoxysuccinyl-L-leucylamido (4-guanidino) butane; N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine

CAS Number: 66701-25-5 Molecular Formula: C15H27N5O5 Molecular Weight: 357.411 g/mol

Beilstein Registry Number: 1405664 MDL Number: MFCD00080261

SKU 02152846-CF
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Description

Product Description

E-64

Application Notes

E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Inhibitor for thiol protease

Specifications
SKU 02152846-CF
Alternate Names [L-3-trans-Carboxyoxiran-2-carbonyl]-L-leucyl-agmatin; trans-Epoxysuccinyl-L-leucylamido (4-guanidino) butane; N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine
Application Notes E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained.
Base Catalog Number 152846
Beilstein Registry Number 1405664
Biochemical Physiological Actions E-64 is an irreversible, potent, and highly selective cysteine protease inhibitor. E-64 does not react with the functional thiol group of non-protease enzymes, such as L-lactate dehydrogenase or creatine kinase. E-64 will not inhibit serine proteases (except trypsin) like other cysteine protease inhibitors, leupeptin and antipain. The trans-epoxysuccinyl group (active moiety) of E-64 irreversibly binds to an active thiol group in many cysteine proteases, such as papain, actinidase, and cathepsins B, H, and L to form a thioether linkage. E-64 is a very useful cysteine protease inhibitor for use in in vivo studies because it has a specific inhibition, it is permeable in cells and tissues and has low toxicity.
CAS # 66701-25-5
Format Powder
Molecular Formula C15H27N5O5
Molecular Weight 357.411 g/mol
Optical Rotation (c=0.5, 0.1 N HCl): +24.9 ° (Lit.)
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
RTECS Number RR0390000
Source Synthetic
Typical Working Concentration 1-10 uM
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.