Deoxyribonuclease I, >50,000 U/mg 10 MU

Deoxyribonuclease I, >50,000 U/mg 10 MU

Synonyms DNase I;Deoxyribonucleate 5′-oligonucleotido-hydrolase; Deoxyribonuclease A

CAS Number: 9003-98-9

EC Number: 232-667-0 MDL Number: MFCD00130918

Packaging size: 10 MU

SKU 0219006210
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Description

Product Description

Deoxyribonuclease I

Application Notes

DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Marker | Cell lysis | Enzymes | Inhibitors | Substrates

Specifications
SKU 0219006210
Alternate Names DNase I;Deoxyribonucleate 5′-oligonucleotido-hydrolase; Deoxyribonuclease A
Application Notes DNase I is used as a genetic marker for forensic identification. Used for the removal of DNA from protein samples. Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis.
Base Catalog Number 19006210
CAS # 9003-98-9
EC Number 232-667-0
Extinction Coefficient 11.1 at 280 (Lit.)
Format Powder
Pack Size 10 MU
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Preparation Method Pancreatic deoxyribonuclease is unusually sensitive to physical denaturation by shaking. Mixing should be done by gentle inversion. Dissolve the standard in 1.0 mL of reagent grade water. Dilute further to a concentration of 20-60 u/mL. All dilutions are made in reagent grade water.
RTECS Number RF0750000
Source Bovine Pancreas
Specific Activity >50,000 Dornase u/mg solid
Typical Working Concentration Dissolve at a concentration of 1 mg/mL. Dilute further to a concentration of 20-60 u/mL immediately before the assay.
Unit Definition Amount of enzyme that causes the fall of 1.0 relative viscosity unit in a solution of highly polymerized DNA in 10 minutes at 30 °C from the intial relative viscosity of 4.0.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.