Creatinine amidohydrolase, 150 units/mg solid, 250 U

Creatinine amidohydrolase, 150 units/mg solid, 250 U

CAS Number: 9025-13-2 Molecular Weight: mol wt ~175 kDa

EC Number: 232-786-8

Packaging size: 250 U

SKU 0215349183
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Specifications
SKU 0215349183
Application Notes This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Base Catalog Number 15349183
Biochemical Physiological Actions Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the beta-strands and the N termini of the main a-helices. These zinc ions indicate the location of the active site.
CAS # 9025-13-2
EC Number 232-786-8
Molecular Weight mol wt ~175 kDa
Pack Size 250 U
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator
pH Optimum pH: 6.5 - 7.5||pH Stability: pH 7.5 - 9.0 (5 deg C, 16hr)
Specific Activity 150 units/mg solid
Unit Definition One unit causes the formation of one umole of creatinine-picrate per minute at pH 7.5 at 37 deg C.
Usage Statement Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.